| Title: | Androgen induces olfactory expression of prostaglandin E(2) receptor Ep1 in the burrow-living fish Bostrychus sinensis |
| Author(s): | Zhang YT; Hong WS; Qiu HT; Wang Q; Chen SX; |
| Address: | "State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Fujian, 361102, PR China. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Fujian, 361102, PR China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian, 361102, PR China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Fujian, 361102, PR China. Electronic address: chenshixi@xmu.edu.cn" |
| Journal Title: | J Steroid Biochem Mol Biol |
| DOI: | 10.1016/j.jsbmb.2019.01.010 |
| ISSN/ISBN: | 1879-1220 (Electronic) 0960-0760 (Linking) |
| Abstract: | "It is well documented that androgens modify olfactory processing in vertebrates. In fish, several lines of evidence indicate that androgens increase olfactory sensitivity to prostaglandin pheromone, but the molecular mechanism is still unclear. Our previous studies showed that prostaglandin E(2) (PGE(2)) is a sex pheromone in the burrowing-living fish Chinese black sleeper (Bostrychus sinensis) and that the PGE(2) receptor 1 (Ep1) in the olfactory rosette is a candidate receptor for sensing sex pheromone PGE(2). In the present study, we found that testosterone (T) and 11-ketotestosterone (11-KT) exhibited stimulatory effects on the expression of ep1 in the olfactory rosette in vivo and ex vivo. Moreover, the androgen receptor (Ar) agonist R1881 had similar effects to 11-KT on the expression of ep1 ex vivo, suggesting the up-regulatory effect is mediated by Ar. The amount of aralpha transcripts ( 1500 copies/100 ng total RNA) was greater than that of arbeta ( 300 copies/100 ng total RNA) in the olfactory rosette, and the expression levels of aralpha increased with spermatogenesis and peaked at late meiosis stage. Moreover, activated Aralpha but not Arbeta transactivated a 2k bp ep1 promoter in HEK293T cell, and some OSNs exhibited co-localization of aralpha mRNA and Ep1 protein signals. Taken together, our results suggest that Aralpha, but not Arbeta, plays a crucial role in mediating the androgen-induced up-regulation of ep1 expression in B. sinensis. The present study is the first to shed light on the molecular mechanisms whereby androgens enhance responsiveness to prostaglandin sex pheromones in teleosts" |
| Keywords: | "Animals HEK293 Cells Humans Male Perciformes/genetics/*physiology RNA, Messenger/genetics Receptors, Prostaglandin E, EP2 Subtype/*genetics Smell Spermatogenesis Testosterone/*analogs & derivatives/*metabolism Transcriptional Activation *Up-Regulation And;" |
| Notes: | "MedlineZhang, Yu Ting Hong, Wan Shu Qiu, Heng Tong Wang, Qiong Chen, Shi Xi eng Research Support, Non-U.S. Gov't England 2019/01/20 J Steroid Biochem Mol Biol. 2019 Apr; 188:156-165. doi: 10.1016/j.jsbmb.2019.01.010. Epub 2019 Jan 16" |
