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Insect Sci

Title:		CRISPR-mediated mutagenesis of the odorant receptor co-receptor (Orco) gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis
Author(s):		Xu L; Jiang HB; Tang KY; Yan Y; Schetelig MF; Wang JJ;
Address:		"Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China. Department of Insect Biotechnology in Plant Protection, Justus-Liebig-University Giessen, Institute for Insect Biotechnology, Giessen, Germany"
Journal Title:		Insect Sci
Year:		2022
Volume:		20220130
Issue:		5
Page Number:		1275 - 1286
DOI:		10.1111/1744-7917.12997
ISSN/ISBN:		1744-7917 (Electronic) 1672-9609 (Linking)
Abstract:		"Olfaction plays an essential role in insect behavior such as host location, foraging, mating, and oviposition. The odorant receptor co-receptor (Orco) is an obligatory odorant receptor and indispensable in odor perception. Here, we characterized the Orco gene from the oriental fruit fly, Bactrocera dorsalis (Hendel), a notorious agriculture pest. The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system. Electroantennograms (EAG) and olfactory preference assays confirmed that BdorOrco(-/-) mutant flies had reduced perception of methyl eugenol, beta-caryophyllene, and ethyl acetate. Oviposition bioassays showed that the eggs laid by BdorOrco(-/-) females mediated by benzothiazole and 1-octen-3-ol were significantly decreased. In addition, BdorOrco(-/-) mutant flies took a significantly longer time to locate the food source compared with wild type (WT) flies. Altogether, our data indicated that Orco is essential for multiple physiological processes in B. dorsalis, and it expands our understanding of the function of insect Orco"
Keywords:		"Animals Benzothiazoles Drosophila Female Insect Proteins/genetics Mutagenesis *Receptors, Odorant/genetics Smell *Tephritidae/genetics CRISPR/Cas9 foraging olfaction oriental fruit fly oviposition;"
Notes:		"MedlineXu, Li Jiang, Hong-Bo Tang, Kai-Yue Yan, Ying Schetelig, Marc F Wang, Jin-Jun eng Australia 2022/01/06 Insect Sci. 2022 Oct; 29(5):1275-1286. doi: 10.1111/1744-7917.12997. Epub 2022 Jan 30"