| Title: | The Mythimna separata general odorant binding protein 2 (MsepGOBP2) is involved in the larval detection of the sex pheromone (Z)-11-hexadecenal |
| Author(s): | Tian Z; Li R; Cheng S; Zhou T; Liu J; |
| Address: | "Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, China. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Mythimna separata is a notorious pest causing crop damages at the larval stage. Gaining insight into larval olfaction mechanisms would provide knowledge for olfaction-based management of M. separata larvae. RESULTS: In the present research, (Z)-11-hexadecenal (Z11-16: Ald), a major component of M. separata sex pheromone, was found to attract early-instar larvae of M. separata in a food context. Using a fluorescent binding assay, we found that M. separata general odorant binding protein 2 (MsepGOBP2) exhibited high binding affinity to Z11-16: Ald. Further, silencing of MsepGOBP2 resulted in a sharp reduction of the response to Z11-16: Ald, which could not be mitigated by increasing the concentration of Z11-16: Ald. Additionally, we employed molecular dynamics-based approaches to unravel the interaction details between MsepGOBP2 and Z11-16: Ald, specifically the binding of Z11-16: Ald to MsepGOBP2. CONCLUSION: Z11-16: Ald is attractive to early-instar larvae of M. separata, and MsepGOBP2 is identified to be indispensable in the larval detection of Z11-16: Ald. These results could aid in the development of olfaction-based methods for controlling M. separata in the larval stage. (c) 2023 Society of Chemical Industry" |
| Keywords: | Animals Larva/metabolism *Moths/physiology *Odorants *Sex Attractants/chemistry *Insect Proteins behavior regulation larval olfaction olfactory proteins oriental armyworm pheromone; |
| Notes: | "MedlineTian, Zhen Li, Ruichi Cheng, Shichang Zhou, Tong Liu, Jiyuan eng Sci-Tech Planning Project of Yangling Demonstration Zone/ Chinese Universities Scientific Fund/ National Natural Science Foundation of China/ Natural Science Foundation of Jiangsu Province/ England 2023/01/22 Pest Manag Sci. 2023 Jun; 79(6):2005-2016. doi: 10.1002/ps.7373. Epub 2023 Feb 14" |
