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Proc Natl Acad Sci U S A

Title:		Identification of the DNA sequences controlling the expression of the MAT alpha locus of yeast
Author(s):		Siliciano PG; Tatchell K;
Address:
Journal Title:		Proc Natl Acad Sci U S A
Year:		1986
Volume:		83
Issue:		8
Page Number:		2320 - 2324
DOI:		10.1073/pnas.83.8.2320
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"We have excised a 28-base-pair DNA fragment from the MAT alpha intergenic region and tested its ability to direct diploid-specific transcriptional repression. This fragment (1643-1671, 5'-GCTTCCCAATGTAGAAAAGTACA-TCATA-3') lies within a region required for the normal diploid-specific repression of the MAT alpha transcripts. First, the fragment was inserted into a 53-base-pair MAT alpha deletion that expresses alpha 1 and alpha 2 constitutively. Insertion of the fragment restores proper diploid regulation to the MAT alpha transcripts: alpha 1 mRNA is strongly repressed and alpha 2 mRNA is reduced by a factor of approximately equal to 10 from its haploid level. The fragment works equally well in either orientation, and two copies of the fragment do not lead to stronger repression than a single copy. We also inserted the fragment at three sites upstream of the CYC1-lacZ fusion gene. Insertions placing the regulatory fragment between the CYC1 upstream activator sequence (UAS) and the coding region make beta-galactosidase efficiently in alpha haploids but produce 1/40th the enzyme in a/alpha diploids. This diploid-specific repression requires functional MATa-1 gene product. Insertion of the MAT fragment on the opposite side of the UAS (37 base pairs upstream of the UAS) also caused diploid repression of the fusion gene, but only by a factor of 7. When the regulatory fragment is inserted at a large distance on the far side of the UAS (375 base pairs), it has little if any effect on beta-galactosidase expression. We postulate that this sequence is the operator recognized by the diploid-specific repressor"
Keywords:		"DNA, Fungal/*genetics DNA, Recombinant Diploidy Gene Expression Regulation *Genes, Fungal *Genes, Regulator Mating Factor Peptides/genetics Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineSiliciano, P G Tatchell, K eng CA37702/CA/NCI NIH HHS/ GM29473/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1986/04/01 Proc Natl Acad Sci U S A. 1986 Apr; 83(8):2320-4. doi: 10.1073/pnas.83.8.2320"