| Title: | Metabolomic analysis of breath volatile organic compounds reveals unique breathprints in children with inflammatory bowel disease: a pilot study |
| Author(s): | Patel N; Alkhouri N; Eng K; Cikach F; Mahajan L; Yan C; Grove D; Rome ES; Lopez R; Dweik RA; |
| Address: | "Department of Pediatric Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH, USA" |
| ISSN/ISBN: | 1365-2036 (Electronic) 0269-2813 (Print) 0269-2813 (Linking) |
| Abstract: | "BACKGROUND: Breath testing is becoming an important diagnostic method to evaluate many disease states. In the light of rising healthcare costs, is important to develop a simple non-invasive tool to potentially identify paediatric patients who need endoscopy for suspected inflammatory bowel disease (IBD). AIM: To analyse exhaled volatile organic compounds (VOCs) and investigate the presence of a unique breath patterns to differentiate paediatric patients with (IBD) from healthy controls. METHODS: A cross-sectional, single-centre study included paediatric IBD patients and healthy controls (age range, 5-21 years). The diagnosis of IBD was confirmed by endoscopic, histological and radiographic data. Exhaled breath was collected and analysed using a selective ion flow tube mass spectroscopy (SIFT-MS) to identify new markers or patterns of IBD. RESULTS: One hundred and seventeen patients (62 with IBD and 55 healthy controls) were included in the study. Linear discriminant analysis and principle component analysis of mass scanning ion peak data demonstrated 21 pre-selected VOCs correctly classify patients with IBD or as healthy controls; P < 0.0001. Multivariable logistic regression analysis further showed three specific VOCs (1-octene, 1-decene, (E)-2-nonene) had excellent accuracy for predicting the presence of IBD with an area under the curve (AUC) of 0.96 (95% CI: 0.93-0.99). No significant difference in VOCs was found between patients with Crohn's disease or ulcerative colitis, and no significant correlation was seen with disease activity. CONCLUSION: These pilot data support the hypothesis that a unique breathprint potentially exists for paediatric IBD in the exhaled metabolome" |
| Keywords: | "Adolescent Adult Alkenes/*analysis/metabolism Biomarkers/analysis/metabolism Breath Tests/methods Child Child, Preschool Colitis, Ulcerative/diagnostic imaging/*metabolism/pathology Crohn Disease/diagnostic imaging/*metabolism/pathology Cross-Sectional St;" |
| Notes: | "MedlinePatel, N Alkhouri, N Eng, K Cikach, F Mahajan, L Yan, C Grove, D Rome, E S Lopez, R Dweik, R A eng P01 HL107147/HL/NHLBI NIH HHS/ RR026231/RR/NCRR NIH HHS/ P01 HL081064/HL/NHLBI NIH HHS/ U01 AA021890/AA/NIAAA NIH HHS/ HL081064/HL/NHLBI NIH HHS/ U10 HL109250/HL/NHLBI NIH HHS/ HL107147/HL/NHLBI NIH HHS/ P01 HL103453/HL/NHLBI NIH HHS/ R21 RR026231/RR/NCRR NIH HHS/ HL109250/HL/NHLBI NIH HHS/ 1U01AA021890/AA/NIAAA NIH HHS/ HL103453/HL/NHLBI NIH HHS/ Research Support, N.I.H., Extramural England 2014/07/22 Aliment Pharmacol Ther. 2014 Sep; 40(5):498-507. doi: 10.1111/apt.12861. Epub 2014 Jul 16" |
