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Sci Rep

Title:		Biochemical and genome sequence analyses of Megasphaera sp. strain DISK18 from dental plaque of a healthy individual reveals commensal lifestyle
Author(s):		Nallabelli N; Patil PP; Pal VK; Singh N; Jain A; Patil PB; Grover V; Korpole S;
Address:		"CSIR-Institute of Microbial Technology, Chandigarh, India. Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"
Journal Title:		Sci Rep
Year:		2016
Volume:		20160921
Issue:
Page Number:		33665 -
DOI:		10.1038/srep33665
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Much of the work in periodontal microbiology in recent years has focused on identifying and understanding periodontal pathogens. As the majority of oral microbes have not yet been isolated in pure form, it is essential to understand the phenotypic characteristics of microbes to decipher their role in oral environment. In this study, strain DISK18 was isolated from gingival sulcus and identified as a Megasphaera species. Although metagenomics studies revealed Megasphaera species as a major group within the oral habitat, they have never been isolated in cultivable form to date. Therefore, we have characterized the DISK18 strain to better understand its role in the periodontal ecosystem. Strain Megasphaera sp. DISK18 displayed the ability to adhere and self-aggregate, which are essential requisite features for inhabiting and persisting in oral cavity. It also coaggregated with other pioneer oral colonizers like Streptococcus and Lactobacillus species but not with Veillonella. This behaviour points towards its role in the ecologic succession of a multispecies biofilm as an early colonizer. The absence of virulence determining genes as observed in whole genome sequence analysis coupled with an inability to degrade collagen reveals that Megasphaera sp. strain DISK18 is likely not a pathogenic species and emphasizes its commensal lifestyle"
Keywords:		Algorithms Bacterial Adhesion Biofilms Dental Plaque/*microbiology Female Humans Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Megasphaera/*chemistry/classification/*genetics/isolation & purification *Metagenome *Metagenomics/methods;
Notes:		"MedlineNallabelli, Nayudu Patil, Prashant P Pal, Vijay Kumar Singh, Namrata Jain, Ashish Patil, Prabhu B Grover, Vishakha Korpole, Suresh eng Research Support, Non-U.S. Gov't England 2016/09/22 Sci Rep. 2016 Sep 21; 6:33665. doi: 10.1038/srep33665"