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New Phytol

Title:		Cell-specific visualization of jasmonates in wounded tomato and Arabidopsis leaves using jasmonate-specific antibodies
Author(s):		Mielke K; Forner S; Kramell R; Conrad U; Hause B;
Address:		"Leibniz-Institut fur Pflanzenbiochemie, D-06120 Halle, Germany. Leibniz-Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany"
Journal Title:		New Phytol
Year:		2011
Volume:		20110125
Issue:		4
Page Number:		1069 - 1080
DOI:		10.1111/j.1469-8137.2010.03638.x
ISSN/ISBN:		1469-8137 (Electronic) 0028-646X (Linking)
Abstract:		"Jasmonates are well-characterized signals in the development of plants and their response to abiotic and biotic stresses, such as touch and wounding by herbivores. A gap in our knowledge on jasmonate-induced processes, however, is the cellular localization of jasmonates. Here, a novel antibody-based approach was developed to visualize jasmonates in cross-sections of plant material. Antibodies raised in rabbits against BSA-coupled jasmonic acid (JA) are specific for JA, its methyl ester and isoleucine conjugate. They do not bind to 12-oxophytodienoic acid, 12-hydoxy-JA or coronatine. These antibodies were used in combination with newly established fixation and embedding methods. Jasmonates were rapidly and uniformly distributed within all cells near the site of damage of a mechanically wounded tomato (Solanum lycopersicum) leaf. Leaf tissue distally located to the wound site exhibited identical distribution, but had a lower signal intensity. The occurrence of jasmonates in all cell types of a wounded leaf was accompanied by transcript accumulation of early JA-induced genes visualized by in situ hybridization. With these new antibodies, a powerful tool is available to detect cell-specifically the occurrence of jasmonates in any jasmonate-dependent stress response or developmental process of plants"
Keywords:		"Antibodies/metabolism Arabidopsis/*cytology/metabolism Cyclopentanes/*analysis Fatty Acids, Unsaturated/analysis Immunohistochemistry/*methods Solanum lycopersicum/*cytology/metabolism Oxylipins/*analysis Plant Growth Regulators/analysis Plant Leaves/*cyt;"
Notes:		"MedlineMielke, Kati Forner, Susanne Kramell, Robert Conrad, Udo Hause, Bettina eng Research Support, Non-U.S. Gov't England 2011/05/13 New Phytol. 2011 Jun; 190(4):1069-1080. doi: 10.1111/j.1469-8137.2010.03638.x. Epub 2011 Jan 25"