| Title: | FAR1 and the G1 phase specificity of cell cycle arrest by mating factor in Saccharomyces cerevisiae |
| Address: | "Rockefeller University, New York, New York 10021, USA" |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "Significant accumulation of Far1p is restricted to the G1 phase of the Saccharomyces cerevisiae cell cycle. Here we demonstrate yeast cell cycle regulation of Far1p proteolysis. Deletions within the 50 N-terminal amino acids of Far1p increase stability and reduce cell cycle regulation of Far1p abundance. Whereas wild-type Far1p specifically and exclusively promotes G1 phase arrest in response to mating factor, stabilized Far1p promoted arrest both during and after G1. The loss of the G1 specificity of Far1p action requires elimination of FAR1 transcriptional regulation (by means of the GAL1 promoter) as well as N-terminal truncation. Thus, the cell cycle specificity of mating factor arrest may be largely due to cell cycle regulation of FAR1 transcription and protein stability" |
| Keywords: | "Amino Acid Sequence Base Sequence *Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins DNA, Fungal/genetics Fungal Proteins/genetics/*physiology G1 Phase/drug effects/physiology Mating Factor Molecular Sequence Data Mutation Peptide Mapping Pep;" |
| Notes: | "MedlineMcKinney, J D Cross, F R eng GM49716/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1995/05/01 Mol Cell Biol. 1995 May; 15(5):2509-16. doi: 10.1128/MCB.15.5.2509" |
