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World J Microbiol Biotechnol

Title:		Antimicrobial activity of endophytic fungi from olive tree leaves
Author(s):		Malhadas C; Malheiro R; Pereira JA; de Pinho PG; Baptista P;
Address:		"REQUIMTE-LAQV, School of Agriculture, Polytechnic Institute of Braganca, Campus de Santa Apolonia, 5300-253, Braganca, Portugal. REQUIMTE/Laboratory of Toxicology, Faculty of Pharmacy, Porto University, Rua Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal. REQUIMTE-LAQV, School of Agriculture, Polytechnic Institute of Braganca, Campus de Santa Apolonia, 5300-253, Braganca, Portugal. pbaptista@ipb.pt"
Journal Title:		World J Microbiol Biotechnol
Year:		2017
Volume:		20170206
Issue:		3
Page Number:		46 -
DOI:		10.1007/s11274-017-2216-7
ISSN/ISBN:		1573-0972 (Electronic) 0959-3993 (Linking)
Abstract:		"In this study, the antimicrobial potential of three fungal endophytes from leaves of Olea europaea L. was evaluated and the host plant extract effect in the antimicrobial activity was examined. The volatile compounds produced by endophytes were identified by GC/MS and further correlated with the antimicrobial activity. In potato dextrose agar, both Penicillium commune and Penicillium canescens were the most effective inhibiting Gram-positive and -negative bacteria (up to 2.7-fold compared to 30 microg/mL chloramphenicol), whereas Alternaria alternata was most effective inhibiting yeasts (up to 8.0-fold compared to 25 mug/mL fluconazole). The presence of aqueous leaf extract in culture medium showed to induce or repress the antimicrobial activity, depending on the endophytic species. In the next step, various organic extracts from both A. alternata mycelium and cultured broth were prepared; being ethyl acetate extracts displayed the widest spectrum of anti-microorganisms at a minimum inhibitory concentration
Keywords:		Acetates/chemistry Alternaria/drug effects Chloramphenicol/pharmacology Endophytes/*metabolism Fluconazole/pharmacology Fungi/*physiology Gram-Negative Bacteria/*drug effects Gram-Positive Bacteria/*drug effects Microbial Sensitivity Tests Olea/chemistry/;
Notes:		"MedlineMalhadas, Cynthia Malheiro, Ricardo Pereira, Jose Alberto de Pinho, Paula Guedes Baptista, Paula eng Germany 2017/02/09 World J Microbiol Biotechnol. 2017 Mar; 33(3):46. doi: 10.1007/s11274-017-2216-7. Epub 2017 Feb 6"