| Title: | Effects of long-term low-dose formaldehyde exposure on global genomic hypomethylation in 16HBE cells |
| Author(s): | Liu Q; Yang L; Gong C; Tao G; Huang H; Liu J; Zhang H; Wu D; Xia B; Hu G; Wang K; Zhuang Z; |
| Address: | "School of Public Health, Sun Yat-sen University, 74 Zhongshan Road 2, Guangzhou 510080, PR China. liuqingcheng222@yahoo.com.cn" |
| DOI: | 10.1016/j.toxlet.2011.05.1039 |
| ISSN/ISBN: | 1879-3169 (Electronic) 0378-4274 (Linking) |
| Abstract: | "Formaldehyde (FA), a volatile organic compound, is a ubiquitous air pollutant that is classified as 'Carcinogenic to humans (Group 1)' by IARC (2006). As a well-recognized human carcinogen, its carcinogenic mechanisms are still poorly understood. Previous studies have emphasized on genetic changes. However, little is known about the epigenetic mechanisms of FA exposure. In this study, We not only characterized the epigenomic response to long-term low-dose FA exposure in 16HBE cells, but also examined the expression of DNA methyltransferases (DNMTs) and the methyl-CpG-binding protein DNA-binding domain protein 2 (MBD2). Each week the 16HBE cells were treated with 10 muM FA for 24 h (h). After 24 weeks (W) of exposure to FA, the level of genomic DNA methylation gradually decreased in a time-related manner. Moreover, our results showed that FA exposure down-regulated the expression of DNMT3a and DNMT3b at both mRNA and protein level, and up-regulated the levels of DNMT1 and MBD2 at both mRNA and protein level. Our study indicated that long-term FA exposure could disrupt genomic DNA methylation, which may be one of the possible underlying carcinogenic mechanisms of FA" |
| Keywords: | 5-Methylcytosine/metabolism Air Pollutants/*toxicity Bronchi/*drug effects/metabolism/pathology Cell Line DNA/chemistry/isolation & purification DNA (Cytosine-5-)-Methyltransferases/genetics/metabolism DNA Methylation/*drug effects DNA-Binding Proteins/ge; |
| Notes: | "MedlineLiu, Qingcheng Yang, Linqing Gong, Chunmei Tao, Gonghua Huang, Haiyan Liu, Jianjun Zhang, Huimin Wu, Desheng Xia, Bo Hu, Gonghua Wang, Kunpeng Zhuang, Zhixiong eng Research Support, Non-U.S. Gov't Netherlands 2011/07/13 Toxicol Lett. 2011 Sep 10; 205(3):235-40. doi: 10.1016/j.toxlet.2011.05.1039. Epub 2011 Jul 1" |
