| Title: | Ethylene and Benzaldehyde Emitted from Postharvest Tomatoes Inhibit Botrytis cinerea via Binding to G-Protein Coupled Receptors and Transmitting with cAMP-Signal Pathway of the Fungus |
| Author(s): | Lin Y; Ruan H; Akutse KS; Lai B; Lin Y; Hou Y; Zhong F; |
| Address: | "State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & College of Horticulture , Fujian Agriculture and Forestry University Fujian , 350013 Fuzhou , Fujian , P. R. China. Institute of Plant Protection , Fujian Academy of Agricultural Sciences , 350013 Fuzhou , Fujian , P. R. China. International Centre of Insect Physiology and Ecology , 30772-00100 Nairobi , Kenya" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Tomato storage conditions are difficult largely due to Botrytis cinerea infection which causes gray mold disease. However, the effects of the volatile organic compounds (VOCs) emitted by postharvest tomatoes on this fungus remain unclear. We analyzed the effects of tomato-emitted VOCs on B. cinerea pathogenicity, germination, and hyphal growth with bioassay, predicted the causative active compounds by principle component analysis, identified G-protein-coupled receptors (GPCRs) which captured chemical signals in the B. cinerea genome by stimulating molecular docking, tested the binding affinities of these receptors for the active compounds by fluorescence binding competition assay, and identified an associated signaling pathway by RNA interfere. The VOCs emitted by postharvest tomatoes inhibited B. cinerea; ethylene and benzaldehyde were the active compounds causing this effect. One of the identified GPCRs in B. cinerea, BcGPR3, bound tightly to both active compounds. Two genes associated with the cAMP signaling pathway (BcRcn1 and BcCnA) were downregulated in wild-type B. cinerea exposed to the active compounds, as well as in the DeltaBcgpr3 B. cinerea mutant. Exposure to postharvest tomato VOCs reduces B. cinerea pathogenicity due to ethylene and benzaldehyde volatiles. The BcGPR3 protein is inactivated by the active compounds, and thus fails to transmit signals to the cAMP pathway, thereby inhibiting B. cinerea" |
| Keywords: | Benzaldehydes/metabolism/*pharmacology Botrytis/*drug effects/genetics/metabolism Cyclic AMP/*metabolism Ethylenes/metabolism/*pharmacology Fungal Proteins/genetics/*metabolism Solanum lycopersicum/*chemistry/metabolism Molecular Docking Simulation Plant; |
| Notes: | "MedlineLin, Yongwen Ruan, Hongchun Akutse, Komivi Senyo Lai, Baochun Lin, Yizhang Hou, Youming Zhong, Fenglin eng 2019/11/07 J Agric Food Chem. 2019 Dec 11; 67(49):13706-13717. doi: 10.1021/acs.jafc.9b05778. Epub 2019 Nov 25" |
