| Title: | Knockdown of microplitis mediator odorant receptor involved in the sensitive detection of two chemicals |
| Author(s): | Li KM; Ren LY; Zhang YJ; Wu KM; Guo YY; |
| Address: | "College of Plant Protection, Northwest A & F University, Yangling, Shannxi, 712100, China" |
| DOI: | 10.1007/s10886-012-0085-y |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Odorant receptors are thought to play critical roles in the perception of chemosensory stimuli by insects. The primary method to address the functions of odorant receptors in insects is to use in vitro binding assays between the receptors and potential chemical stimuli. We injected MmedOrco dsRNA into the abdominal cavity of a braconid wasp, Microplitis mediator, and assayed for expression of this gene 72 h after treatment (RNAi). Quantitative real-time PCR demonstrated that the level of mRNA expression in MmedOrco dsRNA-treated M. mediator was significantly reduced (>90%) when compared with water-treated controls. Furthermore, electroantennogram (EAG) responses of M. mediator to two chemical attractants, nonanal and farnesene, were also reduced significantly (~70%) in RNAi-treated M. mediator when compared to controls. RNAi-treated M. mediator also responded by walking/flying at a lower rate to both chemicals when compared with controls in a Y-tube olfactometer bioassay, which provides direct evidence that MmedOrco plays an important role in perception of nonanal and farnesene in M. mediator" |
| Keywords: | "Aldehydes/metabolism Animals Behavior, Animal Electrophysiological Phenomena Female Flight, Animal Insect Proteins/genetics/*metabolism Male Pheromones/*metabolism RNA Interference RNA, Messenger/genetics Real-Time Polymerase Chain Reaction Receptors, Odo;" |
| Notes: | "MedlineLi, Ke-Ming Ren, Li-Yan Zhang, Yong-Jun Wu, Kong-Ming Guo, Yu-Yuan eng Research Support, Non-U.S. Gov't 2012/03/10 J Chem Ecol. 2012 Mar; 38(3):287-94. doi: 10.1007/s10886-012-0085-y. Epub 2012 Mar 9" |
