| Title: | "Calumenin, a Ca(2+) Binding Protein, Is Required for Dauer Formation in Caenorhabditis elegans" |
| Address: | "Department of Infection Biology, Wonkwang University School of Medicine, Iksan 54538, Republic of Korea. Department of Biology Education, College of Education, Chosun University, Gwangju 61452, Republic of Korea. Department of Biomedical Science, Graduate School, Wonkwang University, Iksan 54538, Republic of Korea. Institute of Wonkwang Medical Science, Wonkwang University, Iksan 54538, Republic of Korea" |
| ISSN/ISBN: | 2079-7737 (Print) 2079-7737 (Electronic) 2079-7737 (Linking) |
| Abstract: | "Caenorhabditis elegans can adapt and survive in dynamically changing environments by the smart and delicate switching of molecular plasticity. C. elegans dauer diapause is a form of phenotypic and developmental plasticity that induces reversible developmental arrest upon environmental cues. An ER (endoplasmic reticulum)-resident Ca(2+) binding protein, calumenin has been reported to function in a variety of malignant diseases in vertebrates and in the process of muscle contraction-relaxation. In C. elegans, CALU-1 is known to function in Ca(2+)-regulated behaviors (pharyngeal pumping and defecation) and cuticle formation. The cuticles of dauer larvae are morphologically distinct from those of larvae that develop in favorable conditions. The structure of the dauer cuticle is thicker and more highly reinforced than that of other larval stages to protect dauer larvae from various environmental insults. Since the calu-1(tm1783) mutant exhibited abnormal cuticle structures such as highly deformed annuli and alae, we investigated whether CALU-1 is involved in dauer formation or not. Ascaroside pheromone (ascr#2) and crude daumone were used under starvation conditions to analyze the rate of dauer formation in the calu-1(tm1783) mutant. Surprisingly, the dauer ratio of the calu-1(tm1783) mutant was extremely low compared to that of the wild type. In fact, the calu-1(tm1783) mutants were mostly unable to enter diapause. We also found that calu-1 is expressed in body-wall muscle and AIA interneurons at the dauer stage. Taken together, our results suggest that CALU-1 is required for normal entry into diapause in C. elegans" |
| Keywords: | C.elegans calcium-binding protein calumenin cuticle dauer; |
| Notes: | "PubMed-not-MEDLINELee, Kyung Eun Cho, Jeong Hoon Song, Hyun-Ok eng NRF-2020R1A2C1013033/National Research Foundation of Korea/ Switzerland 2023/03/30 Biology (Basel). 2023 Mar 17; 12(3):464. doi: 10.3390/biology12030464" |
