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Water Sci Technol

Title:		Microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of PCR-amplified genes
Author(s):		Lee JH; Lee SM; Choi GC; Park HS; Kang DH; Park JJ;
Address:		"Su Engineering Co. Ltd., Yangsan, Korea. ljh@sueng.kr"
Journal Title:		Water Sci Technol
Year:		2011
Volume:		63
Issue:		3
Page Number:		475 - 483
DOI:		10.2166/wst.2011.246
ISSN/ISBN:		0273-1223 (Print) 0273-1223 (Linking)
Abstract:		"Spent sulfidic caustic (SSC) produced from petrochemical plants contains a high concentration of hydrogen sulfide and alkalinity, and some almost non-biodegradable organic compounds such as benzene, toluene, ethylbenzene and xylenes (BTEX). SSC is mainly incinerated with auxiliary fuel, leading to secondary pollution problems. The reuse of this waste is becoming increasingly important from economic and environmental viewpoints. To denitrify wastewater with low COD/N ratio, additional carbon sources are required. Thus, autotrophic denitrification has attracted increasing attention. In this study, SSC was injected as an electron donor for sulfur-based autotrophic denitrification in the modified Ludzack-Ettinger (MLE) process. The efficiencies of nitrification, COD, and total nitrogen (TN) removal were evaluated with varying SSC dosage. Adequate SSC injection exhibited stable autotrophic denitrification. No BTEX were detected in the monitored BTEX concentrations of the effluent. To analyse the microbial community of the MLE process, PCR-DGGE based on 16 S rDNA with EUB primers, TD primers and nirK gene with nirK primers was performed in order to elucidate the application of the MLE process to SSC"
Keywords:		"Autotrophic Processes/*genetics Biodegradation, Environmental Biodiversity Biological Oxygen Demand Analysis Bioreactors/microbiology Caustics/isolation & purification DNA, Ribosomal/genetics Denaturing Gradient Gel Electrophoresis/*methods Denitrificatio;"
Notes:		"MedlineLee, J-H Lee, S-M Choi, G-C Park, H-S Kang, D-H Park, J-J eng Research Support, Non-U.S. Gov't England 2011/02/01 Water Sci Technol. 2011; 63(3):475-83. doi: 10.2166/wst.2011.246"