| Title: | Cytogenetic analyses of the in vitro and in vivo responses of murine cells to peroxyacetyl nitrate (PAN) |
| Author(s): | Kligerman AD; Mottus K; Erexson GL; |
| Address: | "Genetic Toxicology Division, Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711" |
| DOI: | 10.1016/0165-1218(95)90010-1 |
| ISSN/ISBN: | 0027-5107 (Print) 0027-5107 (Linking) |
| Abstract: | "Peroxyacetyl nitrate (PAN) is one of a class of common air pollutant formed by the action of sunlight on volatile organic compounds and nitrogen oxides. PAN has been shown to be a bacterial mutagen. To determine if PAN can cause DNA damage in mammalian cells, we exposed murine peripheral blood lymphocytes (PBLs) to various volumes of PAN in vitro and analyzed the cells for chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and DNA damage using the single cell gel (SCG) assay. At in vitro concentrations of PAN that were cytotoxic (inhibited cell division), an increase in DNA damage was noted in the SCG assay. At lower exposure levels that permitted cell division, no increases in SCEs, CAs, or DNA damage were evident. For in vivo studies, male mice were exposed nose-only by inhalation for 1 h to 0, 15, 39 or 78 ppm PAN, and their lung cells removed and cultured for the scoring of SCEs and CAs. In addition, PBLs and lung cells were analyzed by the SCG assay. No dose-related effects were found in any of the assays. These data indicate that PAN does not appear to be a potent clastogen or DNA damaging agent in mammalian cells in vivo or in vitro" |
| Keywords: | Animals Chromosome Aberrations DNA Damage In Vitro Techniques Lymphocytes Male Mice Mutagenicity Tests Mutagens/*toxicity Peracetic Acid/*analogs & derivatives/toxicity Sister Chromatid Exchange; |
| Notes: | "MedlineKligerman, A D Mottus, K Erexson, G L eng 5T32ES07126/ES/NIEHS NIH HHS/ Research Support, U.S. Gov't, P.H.S. Netherlands 1995/01/01 Mutat Res. 1995 Jan; 341(3):199-206. doi: 10.1016/0165-1218(95)90010-1" |
