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Biol Reprod

Title:		"Effect of prolactin and androgen on the expression of the female-attracting pheromone silefrin in the abdominal gland of the newt, Cynops ensicauda"
Author(s):		Iwata T; Kawahara G; Yamamoto K; Zhou CJ; Nakajo S; Shioda S; Kikuyama S;
Address:		"Department of Biology, School of Education, Waseda University, Shinjuku-ku, Tokyo 169-8050, Japan"
Journal Title:		Biol Reprod
Year:		2000
Volume:		63
Issue:		6
Page Number:		1867 - 1872
DOI:		10.1095/biolreprod63.6.1867
ISSN/ISBN:		0006-3363 (Print) 0006-3363 (Linking)
Abstract:		"Silefrin is a sodefrin-like, female-attracting pheromone comprising 10 amino acids that was isolated from the abdominal gland of the sword-tailed newt, Cynops ensicauda. Hormonal effects on the silefrin precursor mRNA expression and silefrin content in the abdominal gland were investigated in the present study by using Northern blot analysis and radioimmunoassay, respectively. In the abdominal gland of newts treated with prolactin (PRL) plus testosterone propionate (TP), silefrin precursor mRNA expression was markedly enhanced as compared with that in the newts injected with saline, PRL, or TP. Values for radioimmunoassayable silefrin content in the abdominal gland paralleled those for the silefrin precursor mRNA levels. Moreover, silefrin precursor mRNA signals, as revealed by in situ hybridization, as well as stainability of immunoreactive silefrin were much more intense in the epithelial cells of the abdominal gland of the PRL-plus-TP-treated animals than in those of controls. We thus conclude that PRL and androgen are important factors for enhancing silefrin synthesis"
Keywords:		"Androgens/*pharmacology Animals Blotting, Northern Exocrine Glands/drug effects/*metabolism Fluorescent Antibody Technique, Direct In Situ Hybridization Male Oligopeptides/*biosynthesis/genetics Prolactin/*pharmacology RNA, Messenger/biosynthesis Radioimm;"
Notes:		"MedlineIwata, T Kawahara, G Yamamoto, K Zhou, C J Nakajo, S Shioda, S Kikuyama, S eng Research Support, Non-U.S. Gov't 2000/11/25 Biol Reprod. 2000 Dec; 63(6):1867-72. doi: 10.1095/biolreprod63.6.1867"