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Insects

Title:		Expression Levels of Detoxification Enzyme Genes from Dendroctonus armandi (Coleoptera: Curculionidae) Fed on a Solid Diet Containing Pine Phloem and Terpenoids
Author(s):		Dai L; Gao H; Chen H;
Address:		"Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210000, China. College of Forestry, Northwest A&F University, Xianyang 712100, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China"
Journal Title:		Insects
Year:		2021
Volume:		20211011
Issue:		10
Page Number:		-
DOI:		10.3390/insects12100926
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"Bark beetles overcome the toxic terpenoids produced by pine trees by both detoxifying and converting them into a pheromone system. Detoxification enzymes such as cytochrome P450s, glutathione S-transferases, and carboxylesterases are involved in the ability of Dendroctonus armandi to adapt to its chemical environment. Ten genes from these three major classes of detoxification enzymes were selected to study how these enzymes help D. armandi to respond to the host defenses. The expression profile of these detoxification enzyme genes was observed in adult beetles after feeding on different types of diet. Significant differences were observed between two types of seminatural diet containing the phloem of pines, and a purely artificial diet containing five monoterpenes ((-)-alpha-pinene, (-)-beta-pinene, (+)-3-carene, (+/-)-limonene, and turpentine oil) also caused differential transcript levels in the detoxification enzyme genes. The results suggest that monoterpenes enter the beetles through different routes (i.e., respiratory and digestive systems) and cause the expression of different genes in response, which might be involved in pheromone metabolism. In addition, the xenobiotic metabolism in bark beetles should be considered as a system comprising multiple detoxifying enzymes"
Keywords:		Chinese white pine beetle artificial feeding chemical defense cytochrome P450 real-time qPCR;
Notes:		"PubMed-not-MEDLINEDai, Lulu Gao, Haiming Chen, Hui eng 31700572/National Natural Science Foundation of China/ 31870636/National Natural Science Foundation of China/ Switzerland 2021/10/24 Insects. 2021 Oct 11; 12(10):926. doi: 10.3390/insects12100926"