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PLoS One

Title:		Sumoylation of transcription factor Tec1 regulates signaling of mitogen-activated protein kinase pathways in yeast
Author(s):		Wang Y; Abu Irqeba A; Ayalew M; Suntay K;
Address:		"Department of Biology, Saint Louis University, St. Louis, Missouri, United States of America. ywang8@slu.edu"
Journal Title:		PLoS One
Year:		2009
Volume:		20091014
Issue:		10
Page Number:		e7456 -
DOI:		10.1371/journal.pone.0007456
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Tec1 is a transcription factor in the yeast mitogen-activated protein kinase (MAPK) pathway that controls invasive growth. Previously we reported that a fraction of Tec1 protein is sumoylated on residue lysine 54 in normally growing cells. Here we describe regulation and functional consequences of Tec1 sumoylation. We found that activation of Kss1, the MAPK that directly activates Tec1, results in a decrease in Tec1 sumoylation and a concurrent increase of Tec1 transcriptional activity. Consistent with a role of sumoylation in inhibiting Tec1 activity, specifically increasing sumoylation of Tec1 by fusing it to the sumoylating enzyme Ubc9 leads to a dramatic decrease of Tec1 transcriptional activity. Invasive growth is also compromised in Tec1-Ubc9. In contrast, fusing sumoylation-site mutant Tec1, i.e., Tec1(K54R), to Ubc9 did not significantly alter transcriptional activation and had a less effect on invasive growth. Taken together, these findings provide evidence for regulated sumoylation as a mechanism to modulate the activity of Tec1 and validate Ubc9 fusion-directed sumoylation as a useful approach for studying protein sumoylation"
Keywords:		"Biological Assay DNA-Binding Proteins/*metabolism *Gene Expression Regulation, Fungal *MAP Kinase Signaling System Mitogen-Activated Protein Kinases/metabolism Models, Biological Osmolar Concentration Pheromones/metabolism Phosphorylation Protein Binding;"
Notes:		"MedlineWang, Yuqi Abu Irqeba, Ameair Ayalew, Mihretu Suntay, Kristina eng Research Support, Non-U.S. Gov't 2009/10/15 PLoS One. 2009 Oct 14; 4(10):e7456. doi: 10.1371/journal.pone.0007456"