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Nucleic Acids Res

Title:		Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone
Author(s):		Singh A; Chen EY; Lugovoy JM; Chang CN; Hitzeman RA; Seeburg PH;
Address:
Journal Title:		Nucleic Acids Res
Year:		1983
Volume:		11
Issue:		12
Page Number:		4049 - 4063
DOI:		10.1093/nar/11.12.4049
ISSN/ISBN:		0305-1048 (Print) 1362-4962 (Electronic) 0305-1048 (Linking)
Abstract:		"Two genes, MF alpha 1 and MF alpha 2, coding for the alpha-factor in yeast Saccharomyces cerevisiae were identified by in situ colony hybridization of synthetic probes to a yeast genomic library. The probes were designed on the basis of the known amino acid sequence of the tridecapeptide alpha-pheromone. The nucleotide sequence revealed that the two genes, though similar in their overall structure, differ from each other in several striking ways. MF alpha 1 gene contains 4 copies of the coding sequence for the alpha-factor, which are separated by 24 nucleotides encoding the octapeptide Lys-Arg-Glu-Ala-Glu(or Asp)-Ala-Glu-Ala. The first alpha-factor coding block is preceded by a sequence for the hexapeptide Lys-Arg-Glu-Ala and 83 additional amino acids. MF alpha 2 gene contains coding sequences for two copies of the alpha-factor that differ from each other and from alpha-factor encoded by MF alpha 1 gene by a Gln leads to Asn and a Lys leads to Arg substitution. The first copy of the alpha-factor is preceded by a sequence coding for 87 amino acids which ends with Lys-Arg-Glu-Ala-Val-Ala-Asp-Ala. The coding blocks of the two copies of the pheromone are separated by the sequence for Lys-Arg-Glu-Ala-Asn-Ala-Asp-Ala. Thus, the alpha-factor can be derived from 2 different precursor proteins of 165 and 120 amino acids containing, respectively, 4 and 2 copies of the pheromone"
Keywords:		"Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes/metabolism Nucleic Acid Hybridization Pheromones/*genetics Plasmids Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineSingh, A Chen, E Y Lugovoy, J M Chang, C N Hitzeman, R A Seeburg, P H eng England 1983/06/25 Nucleic Acids Res. 1983 Jun 25; 11(12):4049-63. doi: 10.1093/nar/11.12.4049"