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Mol Cell Biol

Title:		The PRE and PQ box are functionally distinct yeast pheromone response elements
Author(s):		Sengupta P; Cochran BH;
Address:		"MIT Center for Cancer Research, Cambridge"
Journal Title:		Mol Cell Biol
Year:		1990
Volume:		10
Issue:		12
Page Number:		6809 - 6812
DOI:		10.1128/mcb.10.12.6809-6812.1990
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Saccharomyces cerevisiae mating pheromones function by binding to cell surface receptors and activating signal transduction processes which regulate gene expression. In this report, we have analyzed the minimum sequence requirements for conferring both a and alpha mating pheromone inducibilities onto a heterologous promoter. Here we show that the repetitive pheromone response element (PRE) which binds to STE12 protein is sufficient to confer pheromone responsiveness only when present in multiple copies. Moreover, by itself, it is preferentially responsive to alpha factor in a cells. In contrast, a single copy of the PQ box of the STE3 upstream activation sequence (UAS) is sufficient to confer a-factor responsiveness in alpha cells. The PQ box binds both MCM1 and MAT alpha 1 in a cooperative manner, and neither the P nor Q site alone is sufficient to confer a-factor responsiveness. In a cells, however, even multiple copies of the PQ box fail to confer alpha-factor responsiveness. Therefore, the PRE and the PQ box are functionally distinct pheromone-responsive elements with opposite cell type specificities. Moreover, these results indicate that the MCM1 protein functions in a signal transduction pathway in a manner analogous to that of its mammalian homolog, the serum response factor, which regulates the expression of the c-fos proto-oncogene in mammals"
Keywords:		"Base Sequence *Genes, Fungal Mating Factor Molecular Sequence Data Oligonucleotide Probes Peptides/*physiology Pheromones/*physiology Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/*genetics/physiology;"
Notes:		"MedlineSengupta, P Cochran, B H eng P01 CA42063/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1990/12/01 Mol Cell Biol. 1990 Dec; 10(12):6809-12. doi: 10.1128/mcb.10.12.6809-6812.1990"