| Title: | Structural basis of the honey bee PBP pheromone and pH-induced conformational change |
| Author(s): | Pesenti ME; Spinelli S; Bezirard V; Briand L; Pernollet JC; Tegoni M; Cambillau C; |
| Address: | "Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Universites de Marseille, Marseille, France" |
| DOI: | 10.1016/j.jmb.2008.04.048 |
| ISSN/ISBN: | 1089-8638 (Electronic) 0022-2836 (Linking) |
| Abstract: | "The behavior of insects and their perception of their surroundings are driven, in a large part, by odorants and pheromones. This is especially true for social insects, such as the honey bee, where the queen controls the development and the caste status of the other individuals. Pheromone perception is a complex phenomenon relying on a cascade of recognition events, initiated in antennae by pheromone recognition by a pheromone-binding protein and finishing with signal transduction at the axon membrane level. With to the objective of deciphering this initial step, we have determined the structures of the bee antennal pheromone-binding protein (ASP1) in the apo form and in complex with the main component of the queen mandibular pheromonal mixture, 9-keto-2(E)-decenoic acid (9-ODA) and with nonpheromonal components. In the apo protein, the C terminus obstructs the binding site. In contrast, ASP1 complexes have different open conformations, depending on the ligand shape, leading to different volumes of the binding cavity. The binding site integrity depends on the C terminus (111-119) conformation, which involves the interplay of two factors; i.e. the presence of a ligand and a low pH. Ligand binding to ASP1 is favored by low pH, opposite to what is observed with other pheromone-binding proteins, such as those of Bombyx mori and Anopheles gambiae" |
| Keywords: | "Amino Acid Sequence Animals Apoproteins/chemistry Bees/*chemistry Carrier Proteins/*chemistry Fatty Acids, Monounsaturated/chemistry Hydrogen-Ion Concentration Insect Proteins/*chemistry Ligands Models, Molecular Molecular Sequence Data Palmitic Acid/chem;" |
| Notes: | "MedlinePesenti, Marion E Spinelli, Silvia Bezirard, Valerie Briand, Loic Pernollet, Jean-Claude Tegoni, Mariella Cambillau, Christian eng Research Support, Non-U.S. Gov't Netherlands 2008/05/30 J Mol Biol. 2008 Jun 27; 380(1):158-69. doi: 10.1016/j.jmb.2008.04.048. Epub 2008 Apr 27" |
