| Title: | Volatiles emission patterns in poplar clones varying in response to ozone |
| Author(s): | Pellegrini E; Cioni PL; Francini A; Lorenzini G; Nali C; Flamini G; |
| Address: | "Department of Tree Science, Entomology and Plant Pathology Giovanni Scaramuzzi, University of Pisa, Pisa, Italy" |
| DOI: | 10.1007/s10886-012-0162-2 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "The volatiles emitted from young and old leaves of two poplar clones (Populus deltoides x maximowiczii, Eridano, and P. x euramericana, I-214) were sampled after exposure to ozone (80 ppb, 5 h d(-1), for 10 consecutive days) by solid phase microextraction and characterized by GC-MS. Only mature leaves of the ozone-sensitive Eridano clone developed necrosis in response to ozone exposure, and their membrane integrity was significantly affected by ozone (+86 and +18 % of levels of thiobarbituric acid reactive substances in mature and young leaves). The headspace of the poplar clones studied here contained mono- and sesquiterpenes, both hydrocarbons and oxygenated ones in Eridano, and only hydrocarbons in the clone I-214. Furthermore, some non-terpenes, such as C(9)-C(15) straight-chain aldehydes and C(12)-C(16) saturated and unsaturated aliphatic hydrocarbons, were detected. Other common non-terpene volatiles were oxygenated aliphatic compounds, mainly C(6)-alcohols and their acetates. Ozone exposure induced a strong change in volatile profiles, depending on clones and leaf age. Regardless of leaf age, in clone I-214, quantities of oxygenated monoterpenes tended to increase after ozone exposure, however, 'O(3) x leaf age' was not significant. In clone Eridano, increases were observed in emissions of hydrocarbons and oxygenated sesquiterpenes in response to ozone treatment. (Z)-3-Hexen-1-ol and (Z)-3-hexenol acetate were present in traces in the headspace of untreated Eridano mature leaves, but quantities slightly increased after ozone treatment. Quantities of non-terpene oxygenated compounds dropped in the headspace of young leaves of both clones (-24 and -44 % in Eridano and I-214) and also in mature ones of I-214 (-50 %) after ozone exposure. Similarly, quantities of non-terpene hydrocarbons in the emissions from mature leaves of both clones (-58 and -49 %, respectively) decreased, while these compounds increased in young leaves of Eridano (+83 %). We suggest that the resistance of the poplar clone I-214 to O(3) is achieved by: i) monoterpenes constitutively present in young leaves and ii) increase of monoterpene content induced by O(3) in mature leaves" |
| Keywords: | "*Ozone Populus/*metabolism *Stress, Physiological Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlinePellegrini, Elisa Cioni, Pier Luigi Francini, Alessandra Lorenzini, Giacomo Nali, Cristina Flamini, Guido eng 2012/07/20 J Chem Ecol. 2012 Jul; 38(7):924-32. doi: 10.1007/s10886-012-0162-2. Epub 2012 Jul 19" |
