| Title: | Volatile compounds from in vitro metabolism of seven Listeria monocytogenes isolates belonging to different clonal complexes |
| Author(s): | Lepe-Balsalobre E; Rubio-Sanchez R; Ubeda C; Lepe JA; |
| Address: | "Department of Clinical Biochemistry, Virgen del Rocio University Hospital, Seville, Spain. Department of Clinical Biochemistry, Valme University Hospital, Seville, Spain. Departamento de Nutricion y Bromatologia, Toxicologia y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor Garcia Gonzalez 2, 41012, Seville, Spain. Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, Infectious Diseases. Virgen del Rocio University Hospital, Institute of Biomedicine of Seville (IBIS), University of Seville/CSIC/University Hospital Virgen del Rocio, Seville, Spain" |
| ISSN/ISBN: | 1473-5644 (Electronic) 0022-2615 (Linking) |
| Abstract: | "Microorganisms produce a wide variety of volatile organic compounds (VOCs) as products of their metabolism and some of them can be specific VOCs linked to the microorganism's identity, which have proved to be helpful for the diagnosis of infection via odour fingerprinting. The aim of this study was to determine the VOCs produced and consumed to characterize the volatile metabolism of seven isolates of different clonal complexes (CCs) of Listeria monocytogenes. For this purpose, dichloromethane extracts from the thioglycolate broth medium were analysed by gas chromatography coupled to mass spectrometry (GC/MS). Also, multivariate analyses were applied to the data obtained. Results showed that all the isolates of L. monocytogenes produced de novo isobutanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 3-(methylthio)-1-propanol, acetic acid, isobutyric acid, butanoic acid, and isovaleric acid. Significant differences were found among isolates for the production amount of these volatiles, which allowed their differentiation. Thus, CC4 (ST-219/CT-3650) and CC87 (ST-87/CT-4557) showed an active volatile compounds metabolism with high consumption nitrogen and sulphur compounds and production of alcohols and acids, and CC8 (ST-8/CT-8813) and CC3 (ST-3/CT-8722) presented a less active volatile metabolism. Moreover, within the VOCs determined, huge differences were found in the production of butanol among the seven isolates analysed, being probably a good biomarker to discriminate among isolates belonging to different CCs. Hence, the analysis of volatile profile generated by the growth of L. monocytogenes in vitro could be a useful tool to differentiate among CCs isolates" |
| Keywords: | Culture Media Gas Chromatography-Mass Spectrometry/methods *Listeria monocytogenes *Volatile Organic Compounds/metabolism Gc-ms Listeria monocytogenes VOCs biomarkers volatile compounds; |
| Notes: | "MedlineLepe-Balsalobre, Esperanza Rubio-Sanchez, Ricardo Ubeda, Cristina Lepe, Jose Antonio eng England 2022/06/21 J Med Microbiol. 2022 Jun; 71(6). doi: 10.1099/jmm.0.001553" |
