| Title: | Quantitative analysis of yeast MAPK signaling networks and crosstalk using a microfluidic device |
| Author(s): | Lee B; Jeong SG; Jin SH; Mishra R; Peter M; Lee CS; Lee SS; |
| Address: | "Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Yuseong-Gu, Daejeon 305-764, Republic of Korea. rhadum@cnu.ac.kr" |
| ISSN/ISBN: | 1473-0189 (Electronic) 1473-0189 (Linking) |
| Abstract: | "Eukaryotic cells developed complex mitogen-activated protein kinase (MAPK) signaling networks to sense their intra- and extracellular environment and respond to various stress conditions. For example, S. cerevisiae uses five distinct MAP kinase pathways to orchestrate meiosis or respond to mating pheromones, osmolarity changes and cell wall stress. Although each MAPK module has been studied individually, the mechanisms underlying crosstalk between signaling pathways remain poorly understood, in part because suitable experimental systems to monitor cellular outputs when applying different signals are lacking. Here, we investigate the yeast MAPK signaling pathways and their crosstalk, taking advantage of a new microfluidic device coupled to quantitative microscopy. We designed specific micropads to trap yeast cells in a single focal plane, and modulate the magnitude of a given stress signal by microfluidic serial dilution while keeping other signaling inputs constant. This approach enabled us to quantify in single cells nuclear relocation of effectors responding to MAPK activation, like Yap1 for oxidative stress, and expression of stress-specific reporter expression, like pSTL1-qV and pFIG1-qV for high-osmolarity or mating pheromone signaling, respectively. Using this quantitative single-cell analysis, we confirmed bimodal behavior of gene expression in response to Hog1 activation, and quantified crosstalk between the pheromone- and cell wall integrity (CWI) signaling pathways. Importantly, we further observed that oxidative stress inhibits pheromone signaling. Mechanistically, this crosstalk is mediated by Pkc1-dependent phosphorylation of the scaffold protein Ste5 on serine 185, which prevents Ste5 recruitment to the plasma membrane" |
| Keywords: | "Adaptor Proteins, Signal Transducing/metabolism *Lab-On-A-Chip Devices Mitogen-Activated Protein Kinases/metabolism *Saccharomyces cerevisiae/genetics/metabolism *Saccharomyces cerevisiae Proteins/genetics/metabolism Signal Transduction;" |
| Notes: | "MedlineLee, Byungjin Jeong, Seong-Geun Jin, Si Hyung Mishra, Ranjan Peter, Matthias Lee, Chang-Soo Lee, Sung Sik eng Research Support, Non-U.S. Gov't England 2020/07/01 Lab Chip. 2020 Aug 7; 20(15):2646-2655. doi: 10.1039/d0lc00203h. Epub 2020 Jun 29" |
