| Title: | "Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis" |
| Author(s): | Keeling CI; Li M; Sandhu HK; Henderson H; Yuen MM; Bohlmann J; |
| Address: | "Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4. Electronic address: ckeeling@alumni.sfu.ca. Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4" |
| DOI: | 10.1016/j.ibmb.2016.01.002 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Bark beetles (Coleoptera: Scolytinae) are pests of many forests around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant pest of western North American pine forests. The MPB is able to overcome the defences of pine trees through pheromone-assisted aggregation that results in a mass attack of host trees. These pheromones, both male and female produced, are believed to be biosynthesized in the midgut and/or fat bodies of these insects. We used metabolite analysis, quantitative proteomics (iTRAQ) and transcriptomics (RNA-seq) to identify proteins and transcripts differentially expressed between sexes and between tissues when treated with juvenile hormone III. Juvenile hormone III induced frontalin biosynthesis in males and trans-verbenol biosynthesis in females, as well as affected the expression of many proteins and transcripts in sex- and tissue-specific ways. Based on these analyses, we identified candidate genes involved in the biosynthesis of frontalin, exo-brevicomin, and trans-verbenol pheromones" |
| Keywords: | Animals Coleoptera/genetics/*metabolism Fat Body/*metabolism Male *Metabolome Pheromones/*biosynthesis *Proteome *Transcriptome Cytochrome P450 Fat bodies Mevalonate pathway Midgut; |
| Notes: | "MedlineKeeling, Christopher I Li, Maria Sandhu, Harpreet K Henderson, Hannah Yuen, Macaire Man Saint Bohlmann, Jorg eng Research Support, Non-U.S. Gov't England 2016/01/23 Insect Biochem Mol Biol. 2016 Mar; 70:170-83. doi: 10.1016/j.ibmb.2016.01.002. Epub 2016 Jan 12" |
