| Title: | Silencing the odorant coreceptor (Orco) disrupts sex pheromonal communication and feeding responses in Blattella germanica: toward an alternative target for controlling insect-transmitted human diseases |
| Author(s): | He P; Ma YF; Wang MM; Wang H; Dewer Y; Abd El-Ghany NM; Chen GL; Yang GQ; Zhang F; He M; |
| Address: | "State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, P. R. China. Bioassay Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Egypt. Pests and Plant Protection Department, Agricultural and Biological Division, National Research Centre, Giza, Egypt. Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, P. R. China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The German cockroach, Blattella germanica, is one of the most severe pests of urban and rural areas. High-throughput genetic screening approaches indicate that the olfactory system of this pest is extremely powerful because it has an extensive array of olfactory receptor genes compared with many other insect species. Several of these genes have been identified previously, but their functions have not yet been characterized. RESULTS: This study describes the sequence of five transcriptomes of B. germanica adult male antennae, female antennae, maxillary palps, legs, and fifth-instar nymph antennae to investigate expression patterns of odorant receptors (ORs). Approximately 90% of ORs were found to be the most highly expressed genes in adult or nymph antennae. Additionally, every OR requires an odorant co-receptor (Orco) to become fully functional, and this was selected and successfully inhibited by injection of the corresponding double-stranded (ds)RNA targeting the Orco. A strong RNA interference (RNAi) effect was observed in which > 75% of Orco messenger RNA (mRNA) was clearly suppressed after 72 h of treatment. Olfactory behavioral assays showed that Orco-impaired B. germanica respond more slowly and show less attraction to one volatile sex pheromone and food resources compared with a control group. CONCLUSION: The results show that Orco plays a pivotal role in both sex pheromone and food-seeking olfactory processes, and provide an alternative genetic technique for controlling this urban pest species by olfactory disruption. (c) 2020 Society of Chemical Industry" |
| Keywords: | "Animals Arthropod Antennae *Blattellidae/genetics Communication Female Humans Insect Proteins/genetics Insecta Male Odorants *Receptors, Odorant/genetics *Sex Attractants German cockroaches RNAi food-seeking behavior odorant receptors olfaction sex pherom;" |
| Notes: | "MedlineHe, Peng Ma, Yun-Feng Wang, Mei-Mei Wang, Hong Dewer, Youssef Abd El-Ghany, Nesreen M Chen, Guang-Lei Yang, Gui-Qing Zhang, Fan He, Ming eng 31860617/National Natural Science Foundation of China/ QKH-J [2019]1109/Natural Science Foundation of Guizhou Province of China/ QKH-J [2020]1Y077/Natural Science Foundation of Guizhou Province of China/ 111 Program/rogram of Introducing Talents of Discipline to Universities of China/ D20023/rogram of Introducing Talents of Discipline to Universities of China/ 2017-33/Scientific Research Foundation of Guizhou University of China/ England 2020/11/19 Pest Manag Sci. 2021 Apr; 77(4):1674-1682. doi: 10.1002/ps.6187. Epub 2020 Nov 26" |
