| Title: | Proteomic analysis of the reproductive organs of the hermaphroditic gastropod Lymnaea stagnalis exposed to different endocrine disrupting chemicals |
| Author(s): | Giusti A; Leprince P; Mazzucchelli G; Thome JP; Lagadic L; Ducrot V; Joaquim-Justo C; |
| Address: | "Laboratory of Animal Ecology and Ecotoxicology, Centre of Analytical Research and Technology (CART), Liege University, Liege, Belgium ; INRA (Institut National de la Recherche Agronomique), UMR0985 Ecologie et Sante des Ecosystemes, Equipe Ecotoxicologie et Qualite des Milieux Aquatiques, Rennes, France. GIGA-Neuroscience, Liege University, Liege, Belgium. Laboratory of Mass Spectrometry, GIGA-R, Liege University, Liege, Belgium. Laboratory of Animal Ecology and Ecotoxicology, Centre of Analytical Research and Technology (CART), Liege University, Liege, Belgium. INRA (Institut National de la Recherche Agronomique), UMR0985 Ecologie et Sante des Ecosystemes, Equipe Ecotoxicologie et Qualite des Milieux Aquatiques, Rennes, France" |
| DOI: | 10.1371/journal.pone.0081086 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Many studies have reported perturbations of mollusc reproduction following exposure to low concentrations (ng/L range) of endocrine disrupting chemicals (EDCs). However, the mechanisms of action of these molecules on molluscs are still poorly understood. Investigation of the modifications of protein expression in organisms exposed to chemicals using proteomic methods can provide a broader and more comprehensive understanding of adverse impacts of pollution on organisms than conventional biochemical biomarkers (e.g., heat-shock proteins, metallothioneins, GST, EROD). In this study we have investigated the impacts of four chemicals, which exhibit different endocrine disrupting properties in vertebrates, on the proteome of the hermaphroditic freshwater pulmonate gastropod Lymnaea stagnalis after 21 days of exposure. Testosterone, tributyltin, chlordecone and cyproterone acetate were chosen as tested compounds as they can induce adverse effects on the reproduction of this snail. The 2D-DIGE method was used to identify proteins whose expression was affected by these compounds. In addition to modifying the expression of proteins involved in the structure and function of the cytoskeleton, chemicals had impacts on the expression of proteins involved in the reproduction of L. stagnalis. Exposure to 19.2 microg/L of chlordecone increased the abundance of ovipostatin, a peptide transmitted during mating through seminal fluid, which reduces oviposition in this species. The expression of yolk ferritin, the vitellogenin equivalent in L. stagnalis, was reduced after exposure to 94.2 ng Sn/L of tributyltin. The identification of yolk ferritin and the modification of its expression in snails exposed to chemicals were refined using western blot analysis. Our results showed that the tested compounds influenced the abundance of yolk ferritin in the reproductive organs. Alteration in proteins involved in reproductive pathways (e.g., ovipostatin and yolk ferritin) could constitute relevant evidence of interaction of EDCs with reproductive pathways that are under the control of the endocrine system of L. stagnalis" |
| Keywords: | Animals Chlordecone/toxicity Cyproterone Acetate/toxicity Endocrine Disruptors/*toxicity Ferritins/metabolism Lymnaea/*drug effects/*metabolism Proteomics/*methods Testosterone/toxicity Trialkyltin Compounds/toxicity; |
| Notes: | "MedlineGiusti, Arnaud Leprince, Pierre Mazzucchelli, Gabriel Thome, Jean-Pierre Lagadic, Laurent Ducrot, Virginie Joaquim-Justo, Celia eng Research Support, Non-U.S. Gov't 2013/12/24 PLoS One. 2013 Nov 19; 8(11):e81086. doi: 10.1371/journal.pone.0081086. eCollection 2013" |
