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J Agric Food Chem

Title:		"Physicochemical Basis and Comparison of Two Type II Sex Pheromone Components Binding with Pheromone-Binding Protein 2 from Tea Geometrid, Ectropis obliqua"
Author(s):		Fu XB; Zhang YL; Qiu YL; Song XM; Wu F; Feng YL; Zhang JY; Li HL;
Address:		"Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences , China Jiliang University , Hangzhou 310018 , China. Hangzhou Tea Research Institute, China Coop. , Hangzhou 310016 , China. Tea Research Institute , Chinese Academy of Agricultural Sciences , Hangzhou 310008 , China"
Journal Title:		J Agric Food Chem
Year:		2018
Volume:		20181206
Issue:		50
Page Number:		13084 - 13095
DOI:		10.1021/acs.jafc.8b04510
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Lepidopteran geometrid moth can produce complex Type II sex pheromone components to attract males and trigger mating behavior. Although several sex pheromone components have been identified, it remains unclear whether their physicochemical roles in sex pheromone sensing are the same. Therefore, we utilized tea geometrid ( Ectropis obliqua) as an example model to investigate and compare the physicochemical basis of two key Type II sex pheromone components, cis-6,7-epoxy-(3Z,9Z)-3,9-octadecadiene ( Z3 Z9-6,7-epo-18:Hy) and ( Z, Z, Z)-3,6,9-octadecatriene (Z3Z6Z9-18:Hy), interacting with pheromone-binding protein 2 ( EoblPBP2) from E. obliqua. Multispectral, thermodynamic, docking, and site-directed mutagenesis indicated that the major sex pheromone component Z3Z9-6,7-epo-18:Hy is more susceptible to pH-tuned than the minor component Z3Z6Z9-18:Hy, whereas Z3Z6Z9-18:Hy seems to be more susceptible to temperature and amino acid mutations than Z3Z9-6,7-epo-18:Hy. Our study suggests that different components of Type II sex pheromone play different binding characters under specific conditions in the physicochemical behavior. This deeply supplements the theoretical knowledge of Type II pheromones involved in the recognition and discrimination in the Lepidopteran sex pheromones family"
Keywords:		Animals Carrier Proteins Female Insect Proteins/*chemistry/genetics/metabolism Male Molecular Docking Simulation Moths/chemistry/genetics/*metabolism Sex Attractants/chemistry/*metabolism Type II sex pheromone molecular docking multispectral and thermodyn;
Notes:		"MedlineFu, Xiao-Bin Zhang, Ya-Li Qiu, Yi-Lei Song, Xin-Mi Wu, Fan Feng, Yi-Lu Zhang, Jian-Yong Li, Hong-Liang eng Comparative Study 2018/11/20 J Agric Food Chem. 2018 Dec 19; 66(50):13084-13095. doi: 10.1021/acs.jafc.8b04510. Epub 2018 Dec 6"