| Title: | Volatilomics Reveals Potential Biomarkers for Identification of Renal Cell Carcinoma: An In Vitro Approach |
| Author(s): | Amaro F; Pinto J; Rocha S; Araujo AM; Miranda-Goncalves V; Jeronimo C; Henrique R; de Lourdes Bastos M; Carvalho M; de Pinho PG; |
| Address: | "UCIBIO, REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Master in Oncology, Institute of Biomedical Sciences Abel Salazar-University of Porto (ICBAS-UP), 4050-313 Porto, Portugal. Cancer Biology & Epigenetics Group, Research Centre (CI-IPOP) Portuguese Oncology Institute of Porto (IPO Porto), 4200-072 Porto, Portugal. Department of Pathology and Molecular Immunology-Biomedical Sciences Institute (ICBAS), University of Porto, 4050-313, Porto, Portugal. Department of Pathology, Portuguese Oncology Institute of Porto (IPO Porto), 4200-072 Porto, Portugal. UFP Energy, Environment and Health Research Unit (FP-ENAS), University Fernando Pessoa, 349, 4249-004 Porto, Portugal" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "The identification of noninvasive biomarkers able to detect renal cell carcinoma (RCC) at an early stage remains an unmet clinical need. The recognition that altered metabolism is a core hallmark of cancer boosted metabolomic studies focused in the search for cancer biomarkers. The present work aims to evaluate the performance of the volatile metabolites present in the extracellular medium to discriminate RCC cell lines with distinct histological subtypes (clear cell and papillary) and metastatic potential from non-tumorigenic renal cells. Hence, volatile organic compounds (VOCs) and volatile carbonyl compounds (VCCs) were extracted by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analysis unveiled a panel of metabolites responsible for the separation between groups, mostly belonging to ketones, alcohols, alkanes and aldehydes classes. Some metabolites were found similarly altered for all RCC cell lines compared to non-tumorigenic cells, namely 2-ethylhexanol, tetradecane, formaldehyde, acetone (increased) and cyclohexanone and acetaldehyde (decreased). Furthermore, significantly altered levels of cyclohexanol, decanal, decane, dodecane and 4-methylbenzaldehyde were observed in all metastatic RCC cell lines when compared with the non-metastatic ones. Moreover, some alterations in the volatile composition were also observed between RCC histological subtypes. Overall, our results demonstrate the potential of volatile profiling for identification of noninvasive candidate biomarkers for early RCC diagnosis" |
| Keywords: | Hs-spme/gc-ms biomarkers cell lines metabolomics renal cell carcinoma volatile compounds; |
| Notes: | "PubMed-not-MEDLINEAmaro, Filipa Pinto, Joana Rocha, Silvia Araujo, Ana Margarida Miranda-Goncalves, Vera Jeronimo, Carmen Henrique, Rui de Lourdes Bastos, Maria Carvalho, Marcia de Pinho, Paula Guedes eng POCI-01-0145-FEDER-030388 - PTDC/SAU-SER/30388/2017/Fundacao para a Ciencia e a Tecnologia/ Switzerland 2020/05/01 Metabolites. 2020 Apr 27; 10(5):174. doi: 10.3390/metabo10050174" |
