| Title: | "Functional characterization of sex pheromone receptors in the purple stem borer, Sesamia inferens (Walker)" |
| Author(s): | Zhang YN; Zhang J; Yan SW; Chang HT; Liu Y; Wang GR; Dong SL; |
| Address: | "Education Ministry, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China; College of Life Sciences, Huaibei Normal University, Huaibei, China" |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "The sex pheromone communication system in moths is highly species-specific and extremely sensitive, and pheromone receptors (PRs) are thought to be the most important factors in males. In the present study, three full-length cDNAs encoding PRs were characterized from Sesamia inferens antennae. These three PRs were all male-specific in expression, but their relative expression levels were very different; SinfOR29 was 17- to 23-fold higher than the other two PRs. Phylogenetic and motif pattern analyses showed that these three PRs were allocated to different PR subfamilies with different motif patterns. Functional analysis using the heterologous expression system of Xenopus oocytes demonstrated that SinfOR29 specifically and sensitively responded to the major pheromone component, Z11-16:OAc [concentration for 50% of maximal effect (EC50 ) = 3.431 x 10(-7) M], while SinfOR21 responded robustly to a minor pheromone component Z11-16:OH (EC50 = 1.087 x 10(-6) M). SinfOR27, however, displayed no response to any of the three pheromone components, but, interestingly, it was sensitive to a non-sex pheromone component Z9,E12-14:OAc (EC50 = 1.522 x 10(-6) M). Our results provide insight into the molecular mechanisms of specificity and sensitivity of the sex pheromone communication system in moths" |
| Keywords: | "Amino Acid Motifs Amino Acid Sequence Animals Arthropod Antennae/metabolism Insect Proteins/chemistry/*genetics/metabolism Male Molecular Sequence Data Moths/*genetics/metabolism Phylogeny Real-Time Polymerase Chain Reaction Receptors, Pheromone/chemistry;" |
| Notes: | "MedlineZhang, Y-N Zhang, J Yan, S-W Chang, H-T Liu, Y Wang, G-R Dong, S-L eng Research Support, Non-U.S. Gov't England 2014/07/22 Insect Mol Biol. 2014 Oct; 23(5):611-20. doi: 10.1111/imb.12109. Epub 2014 Jul 10" |
