« Previous AbstractEffects of ultraviolet and infrared radiation absence or presence on the aroma volatile compounds in winegrape during veraison    Next AbstractImproving production of volatile fatty acids from food waste fermentation by hydrothermal pretreatment »

PLoS One

Title:		"Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)"
Author(s):		Yin J; Feng H; Sun H; Xi J; Cao Y; Li K;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China. jyin@ippcaas.cn"
Journal Title:		PLoS One
Year:		2012
Volume:		20120330
Issue:		3
Page Number:		e33589 -
DOI:		10.1371/journal.pone.0033589
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. Most importantly, trans-11-tetradecen-1-yl acetate, the pheromone component of Loxostege sticticalis, and trans-2-hexenal and cis-3-hexen-1-ol, the most abundant plant volatiles in essential oils extracted from host plants, had high binding affinities to LstiGOBP2 and elicited strong electrophysiological responses from the antennae of adults"
Keywords:		Amino Acid Sequence Animals Arthropod Antennae/physiology Base Sequence Electrodiagnosis/methods Female Fluorescent Dyes/chemistry/metabolism Gene Expression Profiling Ligands Male Molecular Sequence Data Moths/classification/*genetics Open Reading Frames;
Notes:		"MedlineYin, Jiao Feng, Honglin Sun, Hongyan Xi, Jinghui Cao, Yazhong Li, Kebin eng Research Support, Non-U.S. Gov't 2012/04/06 PLoS One. 2012; 7(3):e33589. doi: 10.1371/journal.pone.0033589. Epub 2012 Mar 30"