| Title: | "Characterization and analysis of novel carboxyl/cholinesterase genes possessing the Thr-316 motif in the silkworm, Bombyx mori" |
| Author(s): | Tsubota T; Nakakura T; Shinoda T; Shiotsuki T; |
| Address: | "Invertebrate Gene Function Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 1347-6947 (Electronic) 0916-8451 (Linking) |
| Abstract: | "Juvenile hormone esterases (JHEs) function in juvenile hormone (JH) degradation. In the silkworm, Bombyx mori, we have characterized authentic JHE (Bmjhe) and five other carboxyl/cholinesterase (CCE) genes (Bmcce-1 to -5) with GQSAG, a motif sequence of JHE. But none of the genes appeared to function in vivo as a JHE, except for Bmjhe. Recently it was reported that the GQSAG motif might be dispensable, and that the Thr-316 residue has functional significance for JHE activity. On the basis of these findings, we identified two novel JHE candidates, Bmcce-6 and Bmcce-7, that lack GQSAG but possess Thr-316. In the CCE phylogenetic tree, BmCCE-6 was close to the lepidopteran JHE cluster, while BmCCE-7 constituted the same cluster as pheromone-degrading esterases. The developmental expression profiles were different among Bmjhe, Bmcce-6, and Bmcce-7. None of the proteins hydrolyzed JH in vitro. Our results suggest that only one CCE (BmJHE) functions as JHE in the silkworm" |
| Keywords: | Amino Acid Motifs Animals Bombyx/*enzymology Carboxylic Ester Hydrolases/genetics Cholinesterases/*genetics Phylogeny *Threonine; |
| Notes: | "MedlineTsubota, Takuya Nakakura, Takayo Shinoda, Tetsuro Shiotsuki, Takahiro eng Research Support, Non-U.S. Gov't England 2010/11/13 Biosci Biotechnol Biochem. 2010; 74(11):2259-66. doi: 10.1271/bbb.100480. Epub 2010 Nov 7" |
