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Arch Anim Nutr

Title:		Comprehensive profiling of the metabolome in corn silage inoculated with or without Lactiplantibacillus plantarum using different untargeted metabolomics analyses
Author(s):		Su R; Ke W; Bai J; Wang M; Usman S; Xie D; Xu D; Chen M; Guo X;
Address:		"State Key Laboratory of Grassland Agro-ecosystems, School of Life Sciences, Lanzhou University, Lanzhou, PR China. Probiotics and Biological Feed Research Centre, Lanzhou University, Lanzhou, PR China. Department of Animal Science, Ningxia University, Yinchuan, PR China. College of Grassland Science, Gansu Agricultural University, Lanzhou, China. Department of Animal Science and Technology, Hainan University, Haikou, PR China. State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, PR China"
Journal Title:		Arch Anim Nutr
Year:		2023
Volume:		20230919
Issue:
Page Number:		1 - 19
DOI:		10.1080/1745039X.2023.2247824
ISSN/ISBN:		1477-2817 (Electronic) 1477-2817 (Linking)
Abstract:		"Silage fermentation is a complicated biochemical process involving interactions between microbes and metabolites. However, the overall metabolome feature of ensiled forage and its response to lactic acid bacteria inoculation is poorly understood. Hence, in this study metabolome profiles of whole-plant corn silage inoculated with or without Lactiplantibacillus plantarum were characterised via solid-phase microextraction/gas chromatography/mass spectrometry (SPME-GC-MS), gas chromatography/time-of-flight mass spectrometry (GC-TOF-MS), and Liquid chromatography/Q Exactive HFX mass spectrometry (LC-QE-MS/MS) analysis. There were 2087 identified metabolites including 1143 reliably identified metabolites in fresh and ensiled whole-plant corn. After ensiling, the increased metabolites in whole-plant corn were mainly composed of organic acids, volatile organic compounds (VOC), benzene and substituted derivatives, carboxylic acids and derivatives, fatty acyls, flavonoids, indoles and derivatives, organooxygen compounds (including amines and amides), phenols, pyridines and derivatives, and steroids and steroid derivatives, which includes neurotransmitters and metabolites with aromatic, antioxidant, anti-inflammatory, and antimicrobial activities. Phenylacetaldehyde was the most abundant aromatic metabolite after ensiling. L-isoleucine and oxoproline were the major free amino acids in silage. Ensiling markedly increased the relative abundances of 3-phenyllactic acid, chrysoeriol, 6-O-acetylaustroinulin, acetylcholine, gamma-aminobutyric acid, pyridoxine, and alpha-linoleic acid. Inoculation with L. plantarum remarkably changed silage VOC composition, and essential amino acids, 3-phenyllactic acid, and cinnamaldehyde compared with untreated silage. The present study does not only provide a deeper insight into metabolites of the ensiled whole-plant corn but also reveals metabolites with specific biological functions that could be much helpful in screening novel lactic acid bacteria to well ensile forages. Inoculation with L. plantarum significantly affects the metabolome in ensiled whole-plant corn"
Keywords:		Lactiplantibacillus plantarum Untargeted metabolomics corn silage metabolome;
Notes:		"PublisherSu, Rina Ke, Wencan Bai, Jie Wang, Musen Usman, Samaila Xie, Dongmei Xu, Dongmei Chen, Mengyan Guo, Xusheng eng England 2023/09/20 Arch Anim Nutr. 2023 Sep 19:1-19. doi: 10.1080/1745039X.2023.2247824"