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Mol Genet Genomics

Title:		Microarray and real-time PCR analyses reveal mating type-dependent gene expression in a homothallic fungus
Author(s):		Poggeler S; Nowrousian M; Ringelberg C; Loros JJ; Dunlap JC; Kuck U;
Address:		"Lehrstuhl fur Allgemeine und Molekulare Botanik, Ruhr-Universitat Bochum, 44780, Bochum, Germany. stefanie.poeggeler@rub.de"
Journal Title:		Mol Genet Genomics
Year:		2006
Volume:		20060216
Issue:		5
Page Number:		492 - 503
DOI:		10.1007/s00438-006-0107-y
ISSN/ISBN:		1617-4615 (Print) 1617-4623 (Linking)
Abstract:		"Sordaria macrospora is a homothallic ascomycete which is able to form fertile fruiting bodies without a mating partner. To analyze the molecular basis of homothallism and the role of mating products during fruiting body development, we have deleted the mating type gene Smta-1 encoding a high-mobility group domain (HMG) protein. The DeltaSmta-1 deletion strain is morphologically wild type during vegetative growth, but it is unable to produce perithecia or ascospores. To identify genes expressed under control of Smta-1, we performed a cross-species microarray analysis using Neurospora crassa cDNA microarrays hybridized with S. macrospora targets. We identified 107 genes that are more than twofold up- or down-regulated in the mutant. Functional classification revealed that 81 genes have homologues with known or putative functions. Comparison of array data from DeltaSmta-1 with those from three phenotypically similar mutants revealed that only a limited set of ten genes is deregulated in all mutants. Remarkably, the ppg2 gene encoding a putative lipopeptide pheromone is 500-fold down-regulated in the DeltaSmta-1 mutant while in all other sterile mutants this gene is up-regulated"
Keywords:		"Gene Deletion *Gene Expression Profiling *Genes, Mating Type, Fungal Neurospora crassa/genetics *Oligonucleotide Array Sequence Analysis Phenotype *Polymerase Chain Reaction Reproduction Sordariales/*genetics/growth & development/physiology;"
Notes:		"MedlinePoggeler, S Nowrousian, M Ringelberg, C Loros, J J Dunlap, J C Kuck, U eng P01 GM068087/GM/NIGMS NIH HHS/ R01 GM034985/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Germany 2006/02/17 Mol Genet Genomics. 2006 May; 275(5):492-503. doi: 10.1007/s00438-006-0107-y. Epub 2006 Feb 16"