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Proc Natl Acad Sci U S A

Title:		Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori
Author(s):		Sakurai T; Nakagawa T; Mitsuno H; Mori H; Endo Y; Tanoue S; Yasukochi Y; Touhara K; Nishioka T;
Address:		"Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2004
Volume:		20041115
Issue:		47
Page Number:		16653 - 16658
DOI:		10.1073/pnas.0407596101
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Sex pheromones released by female moths are detected with high specificity and sensitivity in the olfactory sensilla of antennae of conspecific males. Bombykol in the silkmoth Bombyx mori was the first sex pheromone to be identified. Here we identify a male-specific G protein-coupled olfactory receptor gene, B. mori olfactory receptor 1 (BmOR-1), that appears to encode a bombykol receptor. The BmOR-1 gene is located on the Z sex chromosome, has an eight-exon/seven-intron structure, and exhibits male-specific expression in the pheromone receptor neurons of male moth antenna during late pupal and adult stages. Bombykol stimulation of Xenopus laevis oocytes expressing BmOR-1 and BmGalphaq elicited robust dose-dependent inward currents on two-electrode voltage clamp recordings, demonstrating that the binding of bombykol to BmOR-1 leads to the activation of a BmGalphaq-mediated signaling cascade. Antennae of female moths infected with BmOR-1-recombinant baculovirus showed electrophysiological responses to bombykol but not to bombykal. These results provide evidence that BmOR-1 is a G protein-coupled sex pheromone receptor that recognizes bombykol"
Keywords:		"Amino Acid Sequence Animals Base Sequence Bombyx/*genetics/*physiology DNA, Complementary/genetics Female Gene Expression Genes, Insect In Situ Hybridization Insect Proteins/*genetics/*physiology Male Molecular Sequence Data Nucleopolyhedroviruses/genetic;"
Notes:		"MedlineSakurai, Takeshi Nakagawa, Takao Mitsuno, Hidefumi Mori, Hajime Endo, Yasuhisa Tanoue, Shintarou Yasukochi, Yuji Touhara, Kazushige Nishioka, Takaaki eng Research Support, Non-U.S. Gov't 2004/11/17 Proc Natl Acad Sci U S A. 2004 Nov 23; 101(47):16653-8. doi: 10.1073/pnas.0407596101. Epub 2004 Nov 15"