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Anal Chem

Title:		Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles
Author(s):		Barrios-Collado C; Garcia-Gomez D; Zenobi R; Vidal-de-Miguel G; Ibanez AJ; Martinez-Lozano Sinues P;
Address:		"Department of Energy Engineering and Fluid Dynamics, University of Valladolid , 47002 Valladolid, Spain. SEADM S.L., 28036 Madrid, Spain. Department of Chemistry and Applied Biosciences, ETH Zurich , 8093 Zurich, Switzerland. Fossil Ion Technology S.L., 28036 Madrid, Spain. Science Zurich - Zurich PhD Program Molecular Life Sciences, 8093 Zurich, Switzerland"
Journal Title:		Anal Chem
Year:		2016
Volume:		20160204
Issue:		4
Page Number:		2406 - 2412
DOI:		10.1021/acs.analchem.5b04452
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day-night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, approximately 1200 VOCs were monitored displaying different kinetics. To validate the soundness of our in vivo measurements, we fully characterized some key compounds via tandem mass spectrometry (MS/MS) and confirmed their expected behavior based on prior gas chromatography/mass spectrometry (GC/MS) studies. For example, beta-caryophyllene, which is directly related to photosynthesis, was found to show a periodic day-night pattern with highest concentrations during the day. We conclude that the capability of SESI-MS to capture highly dynamic VOC emissions and wide analyte coverage makes it an attractive tool to complement GC/MS in plant studies"
Keywords:		"Begoniaceae/*chemistry/*metabolism Light Molecular Weight Spectrometry, Mass, Electrospray Ionization/instrumentation Time Factors Volatile Organic Compounds/*analysis/metabolism;"
Notes:		"MedlineBarrios-Collado, Cesar Garcia-Gomez, Diego Zenobi, Renato Vidal-de-Miguel, Guillermo Ibanez, Alfredo J Martinez-Lozano Sinues, Pablo eng Research Support, Non-U.S. Gov't 2016/01/28 Anal Chem. 2016 Feb 16; 88(4):2406-12. doi: 10.1021/acs.analchem.5b04452. Epub 2016 Feb 4"