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Biomed Chromatogr

Title:		Microwave-assisted extraction (MAE) combined with gas chromatography-mass spectrometry (GC-MS) for determination of volatile small molecules to evaluate compatibility of antimicrobial peptide PL-5 spray with packaging materials
Author(s):		Liu C; Yu X; Meng Z; Zhao N; Bai F; Zhao L;
Address:		"School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China"
Journal Title:		Biomed Chromatogr
Year:		2020
Volume:		20200112
Issue:		3
Page Number:		e4748 -
DOI:		10.1002/bmc.4748
ISSN/ISBN:		1099-0801 (Electronic) 0269-3879 (Linking)
Abstract:		"A simple and efficient method involving microwave-assisted extraction (MAE) combined with GC-MS was established to determine 1,3-di-tert-butylbenzene (DBB), 2,4-di-tert-butylphenol (DBP), 3,5-di-tert-butyl-4-hydroxybenzaldehyde (DBHBA) for evaluating the compatibility of antimicrobial peptide PL-5 spray with drug-packaging materials. In this study, the antimicrobial peptide PL-5 spray was purified using a Welchrom C18 column, and the high-density polyethylene spray bottle with liquid collection tube was first mixed with absolute ethanol, which would be subjected to MAE for further measurement by GC-MS. Various experimental parameters were systematically optimized, and good linearities were obtained within the range of 0.05-1.00 mug/mL for DBB and DBHBA and 0.50-10.00 mug/mL for DBP, with limits of detection (LODs) of 0.99 ng/mL (DBB), 16.34 ng/mL (DBP), and 1.64 ng/mL (DBHBA). Satisfactory recoveries that ranged from 96.0% to 107.2% were acquired, and the relative standard deviation was
Keywords:		Antimicrobial Cationic Peptides/analysis/*isolation & purification Chemical Fractionation/*methods *Drug Packaging Gas Chromatography-Mass Spectrometry/*methods Limit of Detection Linear Models Microwaves Reproducibility of Results Volatile Organic Compou;
Notes:		"MedlineLiu, Chu Yu, Xiangyong Meng, Zhaorui Zhao, Ning Bai, Fujuan Zhao, Longshan eng ZQN2016011/Yong and Middle-Aged Backbone Personnel Training Program of Shenyang Pharmaceutical University/ 81503029/National Natural Science Foundation of China/ England 2019/11/15 Biomed Chromatogr. 2020 Mar; 34(3):e4748. doi: 10.1002/bmc.4748. Epub 2020 Jan 12"