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Environ Sci Technol

Title:		Dechlorinating microorganisms in a sedimentary rock matrix contaminated with a mixture of VOCs
Author(s):		Lima G; Parker B; Meyer J;
Address:		"School of Engineering, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada. glaucia.lima@utoronto.ca"
Journal Title:		Environ Sci Technol
Year:		2012
Volume:		20120521
Issue:		11
Page Number:		5756 - 5763
DOI:		10.1021/es300214f
ISSN/ISBN:		1520-5851 (Electronic) 0013-936X (Linking)
Abstract:		"Microbiological characterizations of contaminant biodegradation in fractured sedimentary rock have primarily focused on the biomass suspended in groundwater samples and disregarded the biomass attached to fractures and in matrix pores. In fractured sedimentary rock, diffusion causes nearly all contaminant mass to reside in porous, low-permeability matrix. Microorganisms capable of contaminant degradation can grow in the matrix pores if the pores and pore throats are sufficiently large. In this study, the presence of dechlorinating microorganisms in rock matrices was investigated at a site where a fractured, flat-lying, sandstone-dolostone sequence has been contaminated with a mixture of chlorinated and aromatic hydrocarbons for over 40 years. The profile of organic contaminants as well as the distribution and characterization of the microbial community spatial variability was obtained through depth-discrete, high-frequency sampling along a 98-m continuous rock core. Dechlorinating microorganisms, such as Dehalococcoides and Dehalobacter, were detected in the rock matrices away from fracture surfaces, indicating that biodegradation within the rock matrix blocks should be considered as an important component of the system when evaluating the potential for natural attenuation or remediation at similar sedimentary rock sites"
Keywords:		"Bacteria/*genetics Base Sequence Biodegradation, Environmental DNA, Bacterial/analysis/genetics Denaturing Gradient Gel Electrophoresis Genes, Bacterial/genetics Geologic Sediments/*microbiology *Halogenation Microscopy, Electron, Scanning Polymerase Chai;"
Notes:		"MedlineLima, Glaucia Parker, Beth Meyer, Jessica eng Research Support, Non-U.S. Gov't 2012/05/23 Environ Sci Technol. 2012 Jun 5; 46(11):5756-63. doi: 10.1021/es300214f. Epub 2012 May 21"