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Plants (Basel)

Title:		Bioprospecting of Essential Oil-Bearing Plants: Rapid Screening of Volatile Organic Compounds Using Headspace Bubble-in-Drop Single-Drop Microextraction for Gas Chromatography Analysis
Author(s):		Letseka TE; Sepheka NJ; Dubery IA; George MJ;
Address:		"Department of Chemistry and Chemical Technology, National University of Lesotho, P.O. Box 180, Roma 100, Lesotho. Department of Biochemistry, University of Johannesburg, P.O. Box 524, Johannesburg 2006, South Africa"
Journal Title:		Plants (Basel)
Year:		2022
Volume:		20221017
Issue:		20
Page Number:		-
DOI:		10.3390/plants11202749
ISSN/ISBN:		2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking)
Abstract:		"Essential oils are vital constituents of oil-bearing plants. However, their screening still demands harvesting of the plant for laboratory analysis. We report herein a simple, rapid and robust headspace bubble-in-drop microextraction screening technique (BID-SPME) requiring only small amounts of plant material. The optimised method uses 0.5 g of the crushed plant leaves sample obtained in a 2 mL capped chromatography vial, heated to 55 degrees C and sampled with 2 microL heptadecane in a Hamilton gastight syringe equilibrated for 15 min exposed to the headspace volume. The method was applied to three plants, Pinus radiata, Tagetes minuta and Artemisia afra, which are known for their essential oil content. The method was able to extract at least 80% of the oil constituents in such abundance that they could be easily annotated using the gas chromatography-mass spectrometry (GC-MS) mass spectral libraries. The major volatile organic compounds (VOCs) detected included tagetone, terpinen-4-ol, ocimenone, caryophyllene, dihydrotagetone, terpinolene and artemisia ketone, just to mention a few, at different concentrations in different plants. Importantly, these annotated VOCs were also reported in other studies in the same and even different plants, extracted using normal steam distillation and importantly those reported in the literature for different extraction techniques"
Keywords:		Sdme bubble-in-drop essential oils headspace oil-bearing plants volatiles;
Notes:		"PubMed-not-MEDLINELetseka, Thabiso E Sepheka, Ntjana J Dubery, Ian A George, Mosotho J eng Switzerland 2022/10/28 Plants (Basel). 2022 Oct 17; 11(20):2749. doi: 10.3390/plants11202749"