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Environ Sci Technol

Title:		Effect of acute copper sulfate exposure on olfactory responses to amino acids and pheromones in goldfish (Carassius auratus)
Author(s):		Kolmakov NN; Hubbard PC; Lopes O; Canario AV;
Address:		"Centro de Ciencias do Mar, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"
Journal Title:		Environ Sci Technol
Year:		2009
Volume:		43
Issue:		21
Page Number:		8393 - 8399
DOI:		10.1021/es901166m
ISSN/ISBN:		0013-936X (Print) 0013-936X (Linking)
Abstract:		"Exposure of olfactory epithelium to environmentally relevant concentrations of copper disrupts olfaction in fish. To examine the dynamics of recovery at both functional and morphological levels after acute copper exposure, unilateral exposure of goldfish olfactory epithelia to 100 microM CuSO(4) (10 min) was followed by electro-olfactogram (EOG) recording and scanning electron microscopy. Sensitivity to amino acids (l-arginine and l-serine), generally considered food-related odorants, recovered most rapidly (three days), followed by that to catecholamines (3-O-methoxytyramine), bile acids (taurolithocholic acid) and the steroid pheromone, 17,20beta-dihydroxy-4-pregnen-3-one 20-sulfate, which took 28 days to reach full recovery. Sensitivity to the postovulatory pheromone prostaglandin F(2alpha) had not fully recovered even at 28 days. These changes in sensitivity were correlated with changes in the recovery of ciliated and microvillous receptor cell types. Microvillous cells appeared largely unaffected by CuSO(4) treatment. Cilia in ciliated receptor neurones, however, appeared damaged one day post-treatment and were virtually absent after three days but had begun to recover after 14 days. Together, these results support the hypothesis that microvillous receptor neurones detect amino acids whereas ciliated receptor neurones were not functional and are responsible for detection of social stimuli (bile acids and pheromones). Furthermore, differences in sensitivity to copper may be due to different transduction pathways in the different cell types"
Keywords:		Amino Acids/*pharmacology Animals Copper Sulfate/*toxicity Environmental Exposure/analysis Epithelium/drug effects/ultrastructure Goldfish/*physiology Olfactory Receptor Neurons/drug effects/ultrastructure Pheromones/*pharmacology Smell/*drug effects Time;
Notes:		"MedlineKolmakov, Nikolay N Hubbard, Peter C Lopes, Orlando Canario, Adelino V M eng Research Support, Non-U.S. Gov't 2009/11/21 Environ Sci Technol. 2009 Nov 1; 43(21):8393-9. doi: 10.1021/es901166m"