| Title: | The Effects of Cucumber Mosaic Virus and Its 2a and 2b Proteins on Interactions of Tomato Plants with the Aphid Vectors Myzus persicae and Macrosiphum euphorbiae |
| Author(s): | Arinaitwe W; Guyon A; Tungadi TD; Cunniffe NJ; Rhee SJ; Khalaf A; Mhlanga NM; Pate AE; Murphy AM; Carr JP; |
| Address: | "Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK. Alliance of Bioversity International and International Center for Tropical Agriculture (CIAT), Dong Dok, Ban Nongviengkham, Vientiane CB10 1RQ, Laos. Sainsbury Laboratory, Cambridge University, Bateman St, Cambridge CB2 1LR, UK. School of Life Sciences, Keele University, Newcastle ST5 5BG, UK. Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Saffron Walden CB10 1RQ, UK. National Institute for Agricultural Botany-East Malling (NIAB-EMR), West Malling ME19 6BJ, UK" |
| ISSN/ISBN: | 1999-4915 (Electronic) 1999-4915 (Linking) |
| Abstract: | "Cucumber mosaic virus (CMV), a major tomato pathogen, is aphid-vectored in the non-persistent manner. We investigated if CMV-induced volatile organic compounds (VOCs) or other virus-induced cues alter aphid-tomato interactions. Y-tube olfactometry showed that VOCs emitted by plants infected with CMV (strain Fny) attracted generalist (Myzus persicae) and Solanaceae specialist (Macrosiphum euphorbiae) aphids. Myzus persicae preferred settling on infected plants (3 days post-inoculation: dpi) at 1h post-release, but at 9 and 21 dpi, aphids preferentially settled on mock-inoculated plants. Macrosiphum euphorbiae showed no strong preference for mock-inoculated versus infected plants at 3 dpi but settled preferentially on mock-inoculated plants at 9 and 21 dpi. In darkness aphids showed no settling or migration bias towards either mock-inoculated or infected plants. However, tomato VOC blends differed in light and darkness, suggesting aphids respond to a complex mix of olfactory, visual, and other cues influenced by infection. The LS-CMV strain induced no changes in aphid-plant interactions. Experiments using inter-strain recombinant and pseudorecombinant viruses showed that the Fny-CMV 2a and 2b proteins modified tomato interactions with Macrosiphum euphorbiae and Myzus persicae, respectively. The defence signal salicylic acid prevents excessive CMV-induced damage to tomato plants but is not involved in CMV-induced changes in aphid-plant interactions" |
| Keywords: | Animals *Aphids *Cucumovirus/metabolism *Cytomegalovirus Infections *Solanum lycopersicum/metabolism Plant Diseases *Volatile Organic Compounds/metabolism/pharmacology NahG RNA-dependent RNA polymerase epidemiology gas chromatography-mass spectrometry hem; |
| Notes: | "MedlineArinaitwe, Warren Guyon, Alex Tungadi, Trisna D Cunniffe, Nik J Rhee, Sun-Ju Khalaf, Amjad Mhlanga, Netsai M Pate, Adrienne E Murphy, Alex M Carr, John P eng BB/J011762/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom 21ROMITIGATIONFUND CAMBRIDGE BB/W510609/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BB/P023223/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom CONNECTED Network BB/R005397/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BB/F014376/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't Switzerland 2022/08/27 Viruses. 2022 Aug 1; 14(8):1703. doi: 10.3390/v14081703" |
