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PLoS One

Title:		Molecular characterization and differential expression of olfactory genes in the antennae of the black cutworm moth Agrotis ipsilon
Author(s):		Gu SH; Sun L; Yang RN; Wu KM; Guo YY; Li XC; Zhou JJ; Zhang YJ;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom"
Journal Title:		PLoS One
Year:		2014
Volume:		20140801
Issue:		8
Page Number:		e103420 -
DOI:		10.1371/journal.pone.0103420
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Insects use their sensitive and selective olfactory system to detect outside chemical odorants, such as female sex pheromones and host plant volatiles. Several groups of olfactory proteins participate in the odorant detection process, including odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs) and sensory neuron membrane proteins (SNMPs). The identification and functional characterization of these olfactory proteins will enhance our knowledge of the molecular basis of insect chemoreception. In this study, we report the identification and differential expression profiles of these olfactory genes in the black cutworm moth Agrotis ipsilon. In total, 33 OBPs, 12 CSPs, 42 ORs, 24 IRs, 2 SNMPs and 1 gustatory receptor (GR) were annotated from the A. ipsilon antennal transcriptomes, and further RT-PCR and RT-qPCR revealed that 22 OBPs, 3 CSPs, 35 ORs, 14 IRs and the 2 SNMPs are uniquely or primarily expressed in the male and female antennae. Furthermore, one OBP (AipsOBP6) and one CSP (AipsCSP2) were exclusively expressed in the female sex pheromone gland. These antennae-enriched OBPs, CSPs, ORs, IRs and SNMPs were suggested to be responsible for pheromone and general odorant detection and thus could be meaningful target genes for us to study their biological functions in vivo and in vitro"
Keywords:		"Animals Arthropod Antennae/*metabolism Cluster Analysis Computational Biology Female Gene Expression Profiling *Gene Expression Regulation *Genes, Insect Male Membrane Proteins/genetics Molecular Sequence Annotation Moths/*genetics Organ Specificity/genet;"
Notes:		"MedlineGu, Shao-Hua Sun, Liang Yang, Ruo-Nan Wu, Kong-Ming Guo, Yu-Yuan Li, Xian-Chun Zhou, Jing-Jiang Zhang, Yong-Jun eng Research Support, Non-U.S. Gov't 2014/08/02 PLoS One. 2014 Aug 1; 9(8):e103420. doi: 10.1371/journal.pone.0103420. eCollection 2014"