| Title: | "Determination of the pheromone-producing region that has epoxidation activity in the abdominal tip of the Japanese giant looper, Ascotis selenaria cretacea (Lepidoptera: Geometridae)" |
| Author(s): | Fujii T; Suzuki MG; Kawai T; Tsuneizumi K; Ohnishi A; Kurihara M; Matsumoto S; Ando T; |
| Address: | "Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo, University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan" |
| DOI: | 10.1016/j.jinsphys.2006.12.005 |
| ISSN/ISBN: | 0022-1910 (Print) 0022-1910 (Linking) |
| Abstract: | "The epoxydienyl sex pheromone of Ascotis selenaria cretacea can be detected only within a rod-like abdominal tip (RAT) of the female. To clarify which part of the RAT is the sex pheromone-producing region, the RAT was morphologically divided into three sections, defined positionally from the abdomen as sections A, B, and C. GC-MS measurements clearly showed that the sex pheromone compound levels in section B were four times greater than those of the other sections. Microscopic dissection analysis revealed that section B consists of four tissues: rectum, oviduct, musculature, and intersegmental membrane. GC-MS analysis of the individual tissues revealed that approximately 90% of the sex pheromone in section B is localized in the intersegmental membrane. A cell layer was found in the intersegmental membrane after staining with propidium iodide. Furthermore, incubation of tissues dissected from section B with a deuterated trienyl pheromone precursor revealed that the labeled epoxy pheromonal component was detected exclusively in the intersegmental membrane. We have determined that the sex pheromone-producing region of A. s. cretacea is on the terminal side of the intersegmental membrane located between the 8th and 9th abdominal segments" |
| Keywords: | Abdomen Animals Epoxy Compounds/metabolism Female Moths/anatomy & histology/*metabolism Sex Attractants/chemistry/*metabolism; |
| Notes: | "MedlineFujii, Takeshi Suzuki, Masataka G Kawai, Takeshi Tsuneizumi, Kazuhide Ohnishi, Atsushi Kurihara, Masaaki Matsumoto, Shogo Ando, Tetsu eng Research Support, Non-U.S. Gov't England 2007/02/27 J Insect Physiol. 2007 Apr; 53(4):312-8. doi: 10.1016/j.jinsphys.2006.12.005. Epub 2007 Jan 10" |
