| Title: | Identification of soluble binding proteins for an insect neuropeptide |
| Author(s): | Elliott JT; Jurenka RA; Prestwich GD; Roelofs WL; |
| Address: | "Department of Physiology and Biophysics, The University at Stony Brook, New York 11794-8661, USA" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "A photoaffinity analog of Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN) was used to identify PBAN binding proteins in various tissues of the corn earworm moth, H. zea. Synthetic Hez-PBAN was derivatized on Lys-27 with p-benzoyldihydrocinnamoyl-N-hydroxysuccinimide ester (BZDC-NHS ester). The resulting BZDC-PBAN stimulated pheromone production in H. zea isolated abdomens at levels comparable to those of the unmodified peptide. Photoaffinity labeling experiments using [3H]BZDC-PBAN with female moth tissues revealed soluble 100 and 115 kDa proteins in the brain-subesophageal ganglia complex, ventral nerve cord, and thoracic muscle that were specifically labeled with the PBAN analog" |
| Keywords: | "Affinity Labels Amino Acid Sequence Animals Carrier Proteins/*analysis Electrophoresis, Polyacrylamide Gel Female Insect Proteins/biosynthesis/*metabolism/physiology Molecular Sequence Data Moths Neuropeptides/biosynthesis/*metabolism/physiology Photofluo;" |
| Notes: | "MedlineElliott, J T Jurenka, R A Prestwich, G D Roelofs, W L eng AI32498/AI/NIAID NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1997/10/23 Biochem Biophys Res Commun. 1997 Sep 29; 238(3):925-30. doi: 10.1006/bbrc.1997.7410" |
