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Mol Cell Biol

Title:		"Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells"
Author(s):		Spain BH; Bowdish KS; Pacal AR; Staub SF; Koo D; Chang CY; Xie W; Colicelli J;
Address:		"Department of Biological Chemistry and Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095, USA"
Journal Title:		Mol Cell Biol
Year:		1996
Volume:		16
Issue:		12
Page Number:		6698 - 6706
DOI:		10.1128/MCB.16.12.6698
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"We have isolated two novel human cDNAs, gps1-1 and gps2, that suppress lethal G-protein subunit-activating mutations in the pheromone response pathway of the yeast Saccharomyces cerevisiae. Suppression of other pathway-activating events was examined. In wild-type cells, expression of either gps1-1 or gps2 led to enhanced recovery from cell cycle arrest induced by pheromone. Sequence analysis indicated that gps1-1 contains only the carboxy-terminal half of the gps1 coding sequence. The predicted gene product of gps1 has striking similarity to the protein encoded by the Arabidopsis FUS6 (COP11) gene, a negative regulator of light-mediated signal transduction that is known to be essential for normal development. A chimeric construct containing gps1 and FUS6 sequences also suppressed the yeast pheromone pathway, indicating functional conservation between these human and plant genes. In addition, when overexpressed in mammalian cells, gps1 or gps2 potently suppressed a RAS- and mitogen-activated protein kinase-mediated signal and interfered with JNK activity, suggesting that signal repression is part of their normal function. For gps1, these results are consistent with the proposed function of FUS6 (COP11) as a signal transduction repressor in plants"
Keywords:		"Amino Acid Sequence Animals *Arabidopsis Proteins COP9 Signalosome Complex Calcium-Calmodulin-Dependent Protein Kinases/*genetics Cell Line DNA, Complementary/*genetics Fungal Proteins/*genetics GTP-Binding Proteins/*genetics *Gene Expression Regulation H;"
Notes:		"MedlineSpain, B H Bowdish, K S Pacal, A R Staub, S F Koo, D Chang, C Y Xie, W Colicelli, J eng CA 56301/CA/NCI NIH HHS/ GM 24787/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1996/12/01 Mol Cell Biol. 1996 Dec; 16(12):6698-706. doi: 10.1128/MCB.16.12.6698"