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Int J Mol Sci

Title:		PAMP Activity of Cerato-Platanin during Plant Interaction: An -Omic Approach
Author(s):		Luti S; Caselli A; Taiti C; Bazihizina N; Gonnelli C; Mancuso S; Pazzagli L;
Address:		"Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy. simone.luti@unifi.it. Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy. anna.caselli@unifi.it. Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019 Sesto Fiorentino, Italy. cosimo.taiti@unifi.it. Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019 Sesto Fiorentino, Italy. bazihizinanadia@gmail.com. Department of Biology, Università di Firenze, via Micheli 1, 50121 Firenze, Italy. cristina.gonnelli@unifi.it. Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019 Sesto Fiorentino, Italy. stefano.mancuso@unifi.it. Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy. luigia.pazzagli@unifi.it"
Journal Title:		Int J Mol Sci
Year:		2016
Volume:		20160602
Issue:		6
Page Number:		-
DOI:		10.3390/ijms17060866
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Cerato-platanin (CP) is the founder of a fungal protein family consisting in non-catalytic secreted proteins, which work as virulence factors and/or as elicitors of defense responses and systemic resistance, thus acting as PAMPs (pathogen-associated molecular patterns). Moreover, CP has been defined an expansin-like protein showing the ability to weaken cellulose aggregates, like the canonical plant expansins do. Here, we deepen the knowledge on CP PAMP activity by the use of a multi-disciplinary approach: proteomic analysis, VOC (volatile organic compound) measurements, and gas exchange determination. The treatment of Arabidopsis with CP induces a differential profile either in protein expression or in VOC emission, as well changes in photosynthetic activity. In agreement with its role of defense activator, CP treatment induces down-expression of enzymes related to primary metabolism, such as RuBisCO, triosephosphate isomerase, and ATP-synthase, and reduces the photosynthesis rate. Conversely, CP increases expression of defense-related proteins and emission of some VOCs. Interestingly, CP exposure triggered the increase in enzymes involved in GSH metabolism and redox homeostasis (glutathione S-transferase, thioredoxin, Cys-peroxiredoxin, catalase) and in enzymes related to the 'glucosinolate-myrosinase' system, which are the premise for synthesis of defence compounds, such as camalexin and some VOCs, respectively. The presented results are in agreement with the accepted role of CP as a PAMP and greatly increase the knowledge of plant primary defences induced by a purified fungal elicitor"
Keywords:		Disease Resistance Fungal Proteins/*metabolism Genomics *Host-Pathogen Interactions Metabolomics Pathogen-Associated Molecular Pattern Molecules/*metabolism Plant Diseases/microbiology Plants/*metabolism/*microbiology Proteomics Reactive Oxygen Species/me;
Notes:		"MedlineLuti, Simone Caselli, Anna Taiti, Cosimo Bazihizina, Nadia Gonnelli, Cristina Mancuso, Stefano Pazzagli, Luigia eng Switzerland 2016/06/09 Int J Mol Sci. 2016 Jun 2; 17(6):866. doi: 10.3390/ijms17060866"