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J Econ Entomol

Title:		Phenotypic Effects of PBAN RNAi Using Oral Delivery of dsRNA to Corn Earworm (Lepidoptera: Noctuidae) and Tobacco Budworm Larvae
Author(s):		Choi MY; Vander Meer RK;
Address:		"Horticultural Crops Research Unit, USDA-ARS, Corvallis, OR. Center for Medical, Agricultural and Veterinary Entomology (CMAVE), USDA-ARS, Gainesville, FL"
Journal Title:		J Econ Entomol
Year:		2019
Volume:		112
Issue:		1
Page Number:		434 - 439
DOI:		10.1093/jee/toy356
ISSN/ISBN:		1938-291X (Electronic) 0022-0493 (Linking)
Abstract:		"Insect neuropeptides represent more than 90% of all insect hormones. The pheromone biosynthesis activating neuropeptide (PBAN)/pyrokinin family is a major group of insect neuropeptides. These neuropeptides regulate a variety of biological functions from embryo to adult in moths including, sex pheromone biosynthesis and diapause. Other functions are yet to be determined. The identification of suitable target genes is most important for the successful application of RNA interference (RNAi) for pest insect control. Insect neuropeptide genes including PBAN are known to have multiple functions and could be a good target for RNAi suppression. In this study, we selected the PBAN gene and its neuropeptide products as an RNAi target for two economically important moth species, the corn earworm, Helicoverpa zea (Boddie), and the tobacco budworm, Heliothis virescens (Fabricius). We investigated RNAi effects on immature moths that had ingested the specific double-stranded RNA (dsRNA) starting at the first instar larva through pupation. We report that RNAi treatments resulted in delay of larval growth, interference of pupal development, and mortality in the two pest moths. In addition, we selected small interfering RNAs (siRNAs) to determine if they have negative phenotypic effects similar to their full-length RNAi parents. This is one of the few examples of negative RNAi effects on lepidopteran pests via feeding and suggests possible RNAi-based control of pest moths"
Keywords:		"Animals Insect Control/*methods Larva *Moths Neuropeptides/*genetics Phenotype *RNA Interference RNA, Double-Stranded/administration & dosage RNA, Small Interfering;"
Notes:		"MedlineChoi, Man-Yeon Vander Meer, Robert K eng Evaluation Study Research Support, U.S. Gov't, Non-P.H.S. England 2018/12/07 J Econ Entomol. 2019 Feb 12; 112(1):434-439. doi: 10.1093/jee/toy356"