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J Econ Entomol

Title:		Molecular and Binding Characteristics of OBP5 of Bradysia odoriphaga (Diptera: Sciaridae)
Author(s):		Yuting Y; Dengke H; Caihua S; Wen X; Youjun Z;
Address:		"Institute of Insect Sciences, College of Agriculture, Yangtze University, Jingzhou, Hubei Province 434025, China. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China"
Journal Title:		J Econ Entomol
Year:		2021
Volume:		114
Issue:		4
Page Number:		1509 - 1516
DOI:		10.1093/jee/toab095
ISSN/ISBN:		1938-291X (Electronic) 0022-0493 (Linking)
Abstract:		"Odorant-binding proteins (OBPs) capture and transport semiochemicals to olfactory receptors (OR) and function in the fi rst step in insect olfaction. In the present study, we cloned a full-length cDNA sequence of BodoOBP5 from the insect pest Bradysia odoriphaga (Diptera: Sciaridae). Real-time PCR (qRT-PCR) analysis revealed that BodoOBP5 was expressed at higher levels in female adults than in other developmental stages. In the different tissues, BodoOBP5 was highly expressed in the female antennae, whereas low levels were expressed in the head and the male antennae, expression was negligible in other tissues. The recombinant protein of BodoOBP5 was successfully expressed with a bacterial system. Competitive binding assays with nine host plant volatiles and a putative sex pheromone revealed that purified BodoOBP5 strongly bound to two sulfur compounds (methyl allyl disulfide and diallyl disulfide); the corresponding dissolution constants (Ki) were 10.38 and 9.23 muM, respectively. Molecular docking indicated that Leu99, Leu103, Ala143, Tyr107, Phe142, and Trp144 in the hydrophobic cavity of BodoOBP5 are the key residues mediating the interaction of BodoOBP5 with methyl allyl disulfide and diallyl disulfide. RNAi-based Y-tube olfactometer assay indicated that there is no significant difference in methyl allyl disulfide and diallyl disulfide. The results of this study increase our understanding of the binding of BodoOBP5 with plant volatiles, facilitating the development of novel ways to control B. odoriphaga"
Keywords:		"Animals DNA, Complementary *Diptera/genetics Insect Proteins/genetics Molecular Docking Simulation Pheromones Plants *Receptors, Odorant/genetics Bradysia odoriphaga competitive binding assay homology modeling molecular docking odorant-binding protein;"
Notes:		"MedlineYuting, Yang Dengke, Hua Caihua, Shi Wen, Xie Youjun, Zhang eng Research Support, Non-U.S. Gov't England 2021/05/30 J Econ Entomol. 2021 Aug 5; 114(4):1509-1516. doi: 10.1093/jee/toab095"