| Title: | Interglomerular Connectivity within the Canonical and GC-D/Necklace Olfactory Subsystems |
| Author(s): | Uytingco CR; Puche AC; Munger SD; |
| Address: | "Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America. Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland, United States of America. Center for Smell and Taste, University of Florida, Gainesville, Florida, United States of America. Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida, United States of America. Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, University of Florida, Gainesville, Florida, United States of America" |
| DOI: | 10.1371/journal.pone.0165343 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "The mammalian main olfactory system contains several subsystems that differ not only in the receptors they express and the glomerular targets they innervate within the main olfactory bulb (MOB), but also in the strategies they use to process odor information. The canonical main olfactory system employs a combinatorial coding strategy that represents odorant identity as a pattern of glomerular activity. By contrast, the 'GC-D/necklace' olfactory subsystem-formed by olfactory sensory neurons expressing the receptor guanylyl cyclase GC-D and their target necklace glomeruli (NGs) encircling the caudal MOB-is critical for the detection of a small number of semiochemicals that promote the acquisition of food preferences. The formation of these socially-transmitted food preferences requires the animal to integrate information about two types of olfactory stimuli: these specialized social chemosignals and the food odors themselves. However, the neural mechanisms with which the GC-D/necklace subsystem processes this information are unclear. We used stimulus-induced increases in intrinsic fluorescence signals to map functional circuitry associated with NGs and canonical glomeruli (CGs) in the MOB. As expected, CG-associated activity spread laterally through both the glomerular and external plexiform layers associated with activated glomeruli. Activation of CGs or NGs resulted in activity spread between the two types of glomeruli; there was no evidence of preferential connectivity between individual necklace glomeruli. These results support previous anatomical findings that suggest the canonical and GC-D/necklace subsystems are functionally connected and may integrate general odor and semiochemical information in the MOB" |
| Keywords: | "Animals Electric Stimulation Female Flavoproteins/*metabolism GABAergic Neurons/cytology/*physiology Male Mice Mice, Inbred C57BL NADP/*metabolism Olfactory Bulb/cytology/*physiology *Olfactory Pathways Synaptic Transmission/*physiology;neuroscience;" |
| Notes: | "MedlineUytingco, Cedric R Puche, Adam C Munger, Steven D eng R01 DC005633/DC/NIDCD NIH HHS/ R25 GM055036/GM/NIGMS NIH HHS/ T32 GM008181/GM/NIGMS NIH HHS/ T32 NS063391/NS/NINDS NIH HHS/ 2016/12/03 PLoS One. 2016 Nov 30; 11(11):e0165343. doi: 10.1371/journal.pone.0165343. eCollection 2016" |
