| Title: | Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest |
| Author(s): | Uttarotai T; Sutheeworapong S; Crombie AT; Murrell JC; Mhuantong W; Noirungsee N; Wangkarn S; Bovonsombut S; McGenity TJ; Chitov T; |
| Address: | "Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. School of Life Sciences, University of Essex, Colchester CO4 3SQ, UK. School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand. School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK. Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand. Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Environmental Science Research Center (ESRC), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand" |
| ISSN/ISBN: | 2079-7737 (Print) 2079-7737 (Electronic) 2079-7737 (Linking) |
| Abstract: | "Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 x 105 ppbv and 7.2 x 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism" |
| Keywords: | Alcaligenes climate-active gas genome isoprene isoprene degradation; |
| Notes: | "PubMed-not-MEDLINEUttarotai, Toungporn Sutheeworapong, Sawannee Crombie, Andrew T Murrell, J Colin Mhuantong, Wuttichai Noirungsee, Nuttapol Wangkarn, Sunanta Bovonsombut, Sakunnee McGenity, Terry J Chitov, Thararat eng 36/2562/Biodiversity-based Economy Development Office (Public Organisation) (BEDO)/ NE/J009555/1/Natural Environment Research Council/ IsoMet 694578/ERC_/European Research Council/International Switzerland 2022/04/24 Biology (Basel). 2022 Mar 28; 11(4):519. doi: 10.3390/biology11040519" |
